识别肝癌抗原的TCRVβ7亚家族克隆转基因后的生物学作用  

Biological effect of recognizing hepatoma antigen TCRVβ7 subfamily after cloning and transgene

作　　者：陈维春[1] 林月霞[1] 黄树林[1] 周继斌[1] 罗利琼[1] 

机构地区：[1]广东药学院分子生物学研究室,广州510224

出　　处：《中国免疫学杂志》2001年第3期138-141,共4页Chinese Journal of Immunology

基　　金：国家自然科学基金! ( 39770 6 98);广东省自然科学基金!( 380 838);广东省"五个一"工程联合资助! (粤卫号 1996 [2 0 ] )

摘　　要：目的 :研究将TCRVβ7基因转化到淋巴细胞后对肝癌细胞的生物学作用。 方法 :用RT PCR的方法扩增出TCRVβ7基因 ,克隆到表达载体pLXSN上 ,用脂质体转染的方法 ,将重组载体导入淋巴细胞 ,然后将淋巴细胞作用于肝癌细胞 ,淋巴细胞用流式细胞仪测表型 ,肝癌细胞作超微结构分析。结果 :具有TCRVβ7基因表达的淋巴细胞显著增多 ,肝癌细胞出现凋亡。结论Objective:To study the biological effects of TCR to hepatoma cell by transfection Vβ7 to lymphocytes. Methods:TCRVβ7 gene was amplified by RT PCR and cloned to expression vector pLXSN. The recombinant was transferred into lymphocytes by Lipfectin Reagent transfection,then the lymphocytes were co cultured with hepatoma cells.The phenotype of lymphocytes was detected on the Flow Cytometry and the ultrastructure of the hepatoma cells was showed by electronic microscope.Results:The lymphocyte amount with TCRVβ7 expressing in those being transfected was much more than those no transfection.Apoptosis appeared in the hepatoma cells.Conclusion:TCRVβ7 subfamily can recognize hepatoma antigen and stimulate T cell.

关 键 词：TCRVβ7基因 转基因 细胞凋亡 肝癌 抗原 克隆 

分 类 号：R735.7[医药卫生—肿瘤] R730.3[医药卫生—临床医学]