日本血吸虫卵黄铁蛋白DNA免疫在小鼠诱生的保护性免疫力  

INDUCTION OF PROTECTIVE IMMUNITY IN MICE AGAINST SCHISTOSOMA JAPONICUM BY DNA VACCINATION WITH RECOMBINANT PLASMID ENCODING YOLK FERRITIN

作　　者：周俊梅[1] 余新炳[1] 吴忠道[1] 郑亦男[1] 卞国武[1] 

机构地区：[1]中山医科大学寄生虫学研究所,广州510089

出　　处：《中国人兽共患病杂志》2001年第2期11-13,共3页Chinese Journal of Zoonoses

基　　金：中山医科大学"211"工程重点学科建设基金资助

摘　　要：目的　研究日本血吸虫卵黄铁蛋白基因DNA免疫小鼠诱生的保护性免疫力。方法　分两大组进行 ,实验Ⅰ小鼠有预感染 ,实验Ⅱ小鼠没有。将构建的日本血吸虫卵黄铁蛋白基因真核表达重组质粒 pL SjFerl经左后腿肌肉注射BALB/c小鼠 ,共免疫 2次 ,间隔 2周。末次免疫后 5w以血吸虫尾蚴攻击感染 ,6w后计数成虫负荷及肝组织虫卵数和死亡卵百分比。设空质粒免疫组为对照组。结果　PL SjFerl免疫小鼠 ,在实验Ⅰ主要诱生IgG1和IgG3,在实验Ⅱ主要诱生IgG2a和IgG2b ;实验组尚产生较高水平的IgA和IFN γ应答。pL SjFerl免疫小鼠诱生一定程度的减虫率 (2 6 .47% ,34 .0 8% ) ,减卵率(4 0 .0 2 % ,36 .83% )和死亡卵百分比 (2 7.5 0 % ,30 .13% )。结论　PL SjFerl免疫小鼠能产生较明显的保护性免疫力 ,值得进一步深入研究。Aim To investigate the immune efficacy of DNA vaccination in mice against yolk ferritin of Chinese Schistosoma japonicum.Methods Set two-series of immunization experiments.Designed beforehand infection in group Ⅰ,but not in group Ⅱ.BALB/c mice were vaccinated by each quadriceps muscle of left leg with pL-Sj Ferl encoding yolk ferritin gene of Chinese Schistosoma japonicum.Each group was immunized twice with 2wk interval. Mice vaccinated with pLXSN blank vector served as control.Mice were challenged five weeks after final DNA boosting by percutaneous infection with cercariae.Six weeks after infection the mice were perfused,worm burden and eggs in liver,dead eggs were counted.Seta from mice were collected from tail vein at weeks 3,11,17 in group Ⅰand weeks 0,6,12 in group Ⅱ,respectively.Results In groupⅠ,mice produced predominantly IgG1 and IgG3,whereas in group Ⅱ,IgG2a and IgG2b antibodies.Mice vaccinated with pL-SjFerl also produced IgA and IFN-γ.immunization with pL-SjFerl in two group could confer significant worm reduction rate(26.47%,34.08%),egg reduction rate(40.02%,36.83%),and dead egg ratio(27.50%,30.13%).Conclusion DNA vaccination with pL-SjFerl could induce protective immunity in BALB/c mice significantly.It worth further research.

关 键 词：日本血吸虫 卵黄铁蛋白 DNA免疫 保护性免力 小鼠 

分 类 号：R532.21[医药卫生—内科学]