苯白血病患者血清GST总酶活性研究  

Study on GSTase Activity in Benzene Induced Leukemia Patients

作　　者：李昌吉[1] 王文静[2] 龙云芳[1] 詹承烈[1] 许峻峰[1] 

机构地区：[1]华西医科大学附属职业病防治院,四川成都610041 [2]上海市疾病预防控制中心,上海200336

出　　处：《工业卫生与职业病》2001年第2期75-77,共3页Industrial Health and Occupational Diseases

基　　金：卫生部科研基金项目!(96 2 2 0 5 );美国中华医学基金(CMB)资助

摘　　要：应用考马斯亮蓝 (KMSL)法测定苯白血病患者和白血病患者血清GST总酶活性。结果表明 ,苯白血病患者GST总酶活性低于白血病患者和正常对照人群。分层比较发现 ,在GST μ基因缺失的情况下 ,苯白血病组与白血病组、对照组之间GST总酶活性差异有显著性 (P <0 0 1) ,苯白血病患者GST总酶活性显著低于白血病患者和正常人群 ,而且白血病患者GST总酶活性也低于对照组 (P <0 0 5 )。在GSTμ基因携带的情况下 ,苯白血病组与白血病组、对照组之间GST总酶活性的改变不明显。提示 ,由于GST总酶活性降低 ,从而影响了机体对苯及其衍生物的解毒作用 ,GST总酶活性的改变可能是苯致白血病的主要因素之一。GSTμ基因缺失、GST总酶活性的降低与苯白血病的发生有一定的关系。但其相关的程度如何 。GSTs(glutathione S transferases,GSTs/GSTase)activity was determined by KMSL method for 34 benzene induced leukemia patients,27 leukemia patients and 96 controls.GSTase activity in benzene induced leukemia patients was markedly lower than that of leukemia patients and healthy controls.The change in GSTase activity is an important factor in resulting leukemia in case of benzene exposure.By stratified analysis with GST μ gene deletion,GSTase activity in benzene induced leukemia was significantly lower than that in leukemia group and control group(P<0 01),and GSTase activity in leukemia group was lower than that of the control(P<0 05).GST μ gene deletion is an important factor affecting the GSTase activities.Since the soluble glutathione s transferase enzymes have been grouped into five classes,it is of great importance to further determine the activity of the GST μ isoenzyme.

关 键 词：谷胱甘肽巯基转移酶 苯白血病 白血病 

分 类 号：R733.7[医药卫生—肿瘤] R135.12[医药卫生—临床医学]