人卵巢癌裸鼠皮下移植瘤模型的建立  

Human Ovarian Epithelian Adenocarcinoma with Subcutaneous Metastasis Established in Murine Model

作　　者：张长英[1] 成文彩[1] 

机构地区：[1]华中科技大学同济医学院,湖北武汉430032

出　　处：《武汉科技大学学报》2001年第1期97-98,共2页Journal of Wuhan University of Science and Technology

摘　　要：利用人卵巢癌Ｃｏｃｌ细胞株制备裸鼠皮下移植瘤模型。将两种不同浓度Ｃｏｃｌ细胞悬液（５×１０７／１００μＬ及５×１０９／１００μＬ）接种于裸鼠背部皮下，观察３个月后计算各组移植瘤形成率，病理切片及电镜下观察Ｃｏｃｌ细胞及移植瘤细胞。两组移植瘤形成率分别为９３．７５％及１００％。通过病理切片及电镜下观察，细胞株与移植瘤细胞形态无明显差异。Ｃｏｃｌ每注射点细胞浓度为５×１０７／１００μＬ及５×１０９／１００μＬ时成瘤率高，为卵巢癌的进一步研究提供了理想材料和模型。By means of cocl cell strain suspensions,the auth ors have established a model of human ovarian epithelian adenocarcinoma in nude mice.Cocl cells with two different concentrations (5×107/100μL,5×109/100μL) were administrated subcutaneous xenografts in nude mice.After three month observations, the rate of successful inoculation was calculated,electromicroscopic and histopathological examination were conducted.The rates of successful inoculation were 93.75% and 100% in two groups.Cocl cells are not much different with xenograft cells in electromicroscopic and histopathological examination.When the cell concentrations were 5×107/100μL and 5×109/100μL per jection,the rate of successful inoculation is higher,which provides an ideal material and model for further study of human ovarian cancer.

关 键 词：卵巢癌 裸鼠 皮下移植瘤 植瘤形成率 Cocl细胞株 细胞形态 细胞浓度 

分 类 号：R737.31[医药卫生—肿瘤] R－332[医药卫生—临床医学]