运动神经元病患者血清和脑脊液中NSE和GM1抗体测定及其临床意义  

The Significance of NSE and GM1 Antibody in Sera and CSF of Patients with Motor Neuron Disease

作　　者：乔健[1] 陈向军[1] 吕传真[1] Hansotto Reiber 

机构地区：[1]上海医科大学神经病学研究所,上海200040 [2]Laboratory of Neurochemistry

出　　处：《中国神经免疫学和神经病学杂志》2001年第2期109-111,123,共4页Chinese Journal of Neuroimmunology and Neurology

摘　　要：目的　通过测定运动神经元病 (MND)患者血清和脑脊液 (CSF)中神经元特异性烯醇化酶 (NSE)和 GM1抗体 ,初步探讨其临床意义。方法　应用 ELISA法测定 3 0例 MND患者血清和 CSF中 NSE和 GM1抗体水平。结果　 MND组 CSF和血清中 NSE含量均明显高于 NC组 (P<0 .0 1 ,P<0 .0 5) ,而仅在 CSF中 NSE含量高于OND组 (P<0 .0 1 ) ;临床分型以运动神经元损害为主的进行性脊肌萎缩症 (PSMA)患者 CSF中 NSE含量明显高于其他组 (P<0 .0 1 ) ,而血清中 NSE水平在各临床类型间差异并不明显 (P>0 .0 5) ;MND患者血清和 CSF中不论是 Ig M型或是 Ig G型 GM1抗体阳性率均明显高于 OND组和 NC组 (P均 <0 .0 1 ) ;MND患者血清和CSF配对样品 NSE水平有较好的相关性 (r=0 .50 2 ,P<0 .0 1 ) ,MND患者 CSF中 NSE水平与 GM1抗体亦有相关性 (r=0 .4 75,P<0 .0 5) ,其余各组之间均未见明显相关性 (P>0 .0 5)。结论　 MND患者 CSF中 NSE和GM1抗体水平可以反映运动系统神经元损害的程度和范围 ;推测这种神经元的损伤可能与Objective To study the levels of neuron-specific enolase (NSE) and antibody against GM1 ganglioside in sera and CSF of patients with motor neuron disease(MND) and to evaluate their clinical significance. Methods The levels of NSE and GM1 antibody in sera and CSF of 30 patients with MND were analyzed with enzyme-linked immunosorbent assays (ELISA). Results The levels of NSE in both CSF and sera of MND pati-ents were significantly higher than normal control(NC) group(P<0.01,P<0.05 respectively), only in CSF the NSE level of MND group was significantly higher than other neurological diseases (OND) group(P<0.01); Among different types of MND, the NSE level in CSF of PSMA type was significantly higher than the other types(P<0.01),while it was not the case of sera(P>0.05); In both CSF and sera, the positivity of IgM-and IgG-GM1 antibody of MND pati-ents were significantly higher than OND and NC group(P<0.01);In addition, the NSE level in the CSF was closely correlated to that in serum of MND patients(r=0.502, P<0.01),and there was also a significant correlation between NSE level and GM1 antibody in CSF of MND patients (r= 0.475, P<0.05). Conclusions The levels of NSE and GM1 antibody in CSF may reflect the extent and territory of the neuron damage in motor system of patients with MND; We speculate that this damage might be related tothe GM1 antibody-mediated humoral immune responses in central nervous system.

关 键 词：运动神经元疾病 神经元特异性烯醇化酶 GM1抗体 

分 类 号：R744.8[医药卫生—神经病学与精神病学]