碱性成纤维细胞生长因子对牙周细胞生物学活性的影响  

Effects of bFGF on Biological Characteristics of Cells Derived from Periodontium

作　　者：葛少华[1] 杨丕山[1] 

机构地区：[1]山东医科大学附属口腔医院,山东济南250012

出　　处：《上海口腔医学》2001年第1期24-26,共3页Shanghai Journal of Stomatology

基　　金：山东省自然科学基金!(Y98C0 80 3 7)

摘　　要：目的　观察基因重组人碱性成纤维细胞生长因子 (rh bFGF)对人牙龈成纤维细胞 (GF)、人牙周韧带成纤维细胞 (PDLF)及人牙槽骨细胞 (ABC)的增殖、碱性磷酸酶活性、总蛋白含量及对 3种细胞矿化结节形成能力的影响。方法　采用细胞培养、MTT比色测定、碱性磷酸酶测定法、考马斯亮蓝法及茜素红染色法。结果　bFGF能促进 3种细胞的增殖 ,但对PDLF和ABC的ALP活性 ,蛋白含量及矿化结节的形成有抑制作用。结论　bFGF可促进细胞的增殖 ,抑制细胞的分化成熟 ,从而促进牙周再生。Objective To observe effects of basic fibroblast growth factor on the biological characteristics of cultured human gingival,periodontal ligament fibroblasts and human alveolar bone cells, such as proliferation, alkaline phosphatase activity, protein synthesis and the formation of mineralized nodules. Methods Using cell culture technique, MTT colorimetric assay, ALP activity assay, Commasie brilliant blue staining and Dahl McGec Russell's alizarin red stain for calcium.Results bFGF enhanced the proliforative responses of the three types of cells. In contrast, bFGF inhabited the induction of alkaline phosphatase activity, protein synthesis and the mineralized nodule formation by PDLF and ABC. Conclusion bFGF can enhance cell proliferation while inhibit cytodifferentiation, thus accelerating periodontal regeneration. [

关 键 词：碱性成纤维细胞生长因子 牙龈成纤维细胞 牙周韧带成纤维细胞 牙槽骨细胞 增殖与分化 

分 类 号：R780.2[医药卫生—口腔医学]