4NQO诱发大鼠舌癌变细胞分化异常的超微结构观察  

Ultramicrostructural Observation on Epithelial Cell disdifferentiation in Rat Tongue Carcinogenesis Induced by 4NQO

作　　者：刘兴坤[1] 何荣根[2] 陈万涛[2] 张志愿[2] 周曾同[2] 周晓健[2] 

机构地区：[1]苏州市口腔医院口腔颌面外科,江苏苏州215005 [2]上海第二医科大学附属第九人民医院口腔医学院口腔颌面外科,上海200011

出　　处：《上海口腔医学》2001年第1期43-45,共3页Shanghai Journal of Stomatology

基　　金：国家自然科学基金!( 3 9670 787)

摘　　要：目的　探讨 4 硝基喹啉 1 氧化物 (4NQO)诱发大鼠舌癌变细胞分化异常的超微结构及其变化规律。方法　口服 4NQO诱发大鼠舌粘膜癌变 ,透射电镜动态观察癌变过程中细胞的超微结构变化。结果　随大鼠舌癌变进展 ,鳞状上皮细胞浆、核、细胞间连接、基底膜等发生规律性变化。其中张力原纤维、桥粒、角质颗粒呈逐渐减少趋势 ,线粒体则逐渐增多 ;部分重度异常增生上皮已发生基底膜断裂 ,原位癌细胞有个别已突破基底膜。结论　口腔癌变是一个渐变过程 ,鳞状上皮分化异常主要表现为合成产物减少和增殖代谢活跃细胞器增多。电镜观察基底膜可以早期发现癌变。Objective To clarify epithelial cell ultramicrostructure changes in oral carcinogenesis. Method The epithelial cell ultramicrostructures in tongue carcinogenesis induced by 4 nitroquinoline 1 oxide (4NQO) were observed by transmission electron microscope. Results With the progress of oral carcinogenesis, regular changes of epithelial cell were showed: decrease in tonofibril, keratohyaline granules, desmosome; and increase in mitochondria. The basement membrane was broken in some severe dysplasia (sDP) and was broken through by parts of cell processes in in situ carcinoma (ISC). Conclusion Oral carcinogenesis is a multistep process. Epithelial cell aberrant differentiation is showed by a decrease in the synthetic products of epithelium cell. [

关 键 词：舌癌 4NQO 分化 大鼠 超微结构 

分 类 号：R739.8[医药卫生—肿瘤]