变形链球菌和远缘链球菌耐氟菌株DNAG+C含量测定  

Base Composition of DNA from Fluoride Resistant Strains of Streptococcus Mutans and Streptococcus Sobrinus

作　　者：盛江筠[1] 刘正[1] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔医学院口腔内科,上海200011

出　　处：《上海口腔医学》2001年第1期49-51,共3页Shanghai Journal of Stomatology

基　　金：国家自然科学基金!( 3 9870 780 )

摘　　要：目的　探讨变形链球菌和远缘链球菌耐氟菌株基因型是否发生改变。方法　采用高效液相色谱法测定变形链球菌和远缘链球菌及相应耐氟菌株DNA碱基含量 ,即DNAG +C摩尔百分比 (mol% )。结果　四株耐氟菌株DNAG +Cmol%为 5 4.45 ,44 .86 ,5 4.41,5 5 .39;相应亲代菌株G +Cmol%为 35 .8,37.4,43.35和 46 .6 2。两者明显不同 ,差值大于 5 %。结论　变形链球菌和远缘链球菌的耐氟菌株已发生基因型改变 ,且可能包括多个基因的突变。Objective To evaluate the genotype change of fluoride resistant strains of S.mutans and S.sobrinus. Methods High potential liquid chromatography was used to measure the base composition of DNA from F resistant strains of S.mutans and S.sobrinus and their parent strains. Results The data showed that the DNA G+C mol% of F resistant and F sensitive S.mutans were quite different, the difference was over 5%. The four F resistant strains were 54.45,44.86,54.41,55,390, whereas the corresponding four parent strains were 35.38,37.4,43.35,46.62, respectively. Conclusion The genotypes of F resitant strains of S.mutans and S.sobrinus have been changed and may comprise several gene mutations. [

关 键 词：耐氟菌株 变形链球菌 远缘链球菌 DNAG+Cmol% 基因型 口腔科 

分 类 号：R780.2[医药卫生—口腔医学]