应用流式细胞技术分析Ca(OH)_2及HA对牙髓细胞周期的影响  

Analysis of the Influence of Ca(OH)_2 and HA on Cell Cycle of Pulp Cells by Flow Cytometry

作　　者：张金宁[1] 王晓仪[1] 

机构地区：[1]上海第二医科大学附属第九人民医院,上海200011

出　　处：《上海口腔医学》2001年第1期52-53,共2页Shanghai Journal of Stomatology

摘　　要：目的　采用流式细胞技术分析盖髓剂氢氧化钙 [Ca(OH2 ) ]及羟基磷灰石 (HA)对牙髓细胞周期的影响。方法　将香猪恒牙牙髓机械暴露后 ,行氢氧化钙和羟基磷灰石盖髓术 ,分别于术后 2 ,4,8,12周拔牙 ,采取牙髓 ,胶原酶消化后流式细胞仪检测。将组方图存入磁盘进行计算机分析处理 ,得到G0 /G1,G2 ,S及M期细胞百分比。结果　氢氧化钙和羟基磷灰石均能使牙髓细胞的G0 /G1期细胞减少 ,S期细胞积累。结论　提示氢氧化钙及羟基磷灰石可能有促进G0 /G1期细胞进入S期 ,增加DNA合成的作用。Objective The influence of Ca(OH) 2 (calcium hydroxide) and HA(hydroxyapatite) as pulp capping agents on cell cycle of pulp cells was analyzed by flow cytometry. Method Permanent teeth pulps of pigs were capped with Ca(OH) 2, and HA following mechanical pulp exposure. The teeth were extracted after 2, 4, 8 and 12 weeks, respectively. The pulps were taken and digested by collagenase. The percentages of pulp cells in G0/G1,G2,S and M periods were determined by flow cytometry and computer analysis. Results The percentage of cells in G0/G1, period was decreased, whereas the percentage of cells in S period was increased after pulp capping with Ca(OH) 2 and HA. Conclusion This study indicated that Ca(OH) 2 and HA could increase DNA synthesis of pulp cells. [

关 键 词：流式细胞技术 细胞周期 氢氧化钙 羟基磷灰石 

分 类 号：R781.05[医药卫生—口腔医学]