利什曼原虫中国分离株SSU rDNA多变区序列分析  

SEQUENCE ANALYSIS OF SSU rDNA VARIABLE REGION OF DIFFERENT LEISHMANIA ISOLATES OF CHINA

作　　者：卜玲毅[1] 胡孝素[1] 敬保迁[2] 马莹[1] 易桃林[1] 

机构地区：[1]华西医科大学寄生虫学研究室,四川成都610044 [2]川北医学院微生物学教研室,四川南充637000

出　　处：《实用寄生虫病杂志》2001年第1期1-3,共3页Journal of Practical Parasitic Diseases

基　　金：国家自然科学基金资助项目!(3 9970 667)&&

摘　　要：目的分析我国荒漠、山丘疫区利什曼原虫分离株的 SSU r DNA多变区序列差异。方法 n DNA进行PCR扩增 ,将扩增出的 SSU r DNA基因的特异片段克隆于 p GEMR-T Easy Vector上 ,采用通用引物 M1 3进行 PCR扩增 ,全自动测序仪测序。结果序列分析显示本文报道的荒漠、山丘疫区的 2株利什曼原虫 (L.d.XJ771、L.d.SC6)的 SSU r DNA序列大小均为 3 92 bp;序列差异发生在两个独特序列区 (UQ- 和 UQ- ) ,无移码突变 ;与 Gene Bank中的利什曼原虫比较分析 ,同源性在 98%以上。结论我国荒漠、山丘疫区利什曼原虫分离株之间的 SSU r DNA多变区的碱基序列有差异 ;荒漠疫区分离株 L.d.XJ771与国际标准株 L.d.DD8的 SSU rAim To analyze the sequence of the SSU rDNA variable region of Leishmania isolates from desert and hill foci of China. Methods Specific SSU rDNA fragments from nuclear DNA of two Leishmania isolates were amplified by PCR and then cloned into pGEMR-T Easy Vector.After that, the specific fragments were sequenced by the automated DNA sequencer. Results Sequence analysis showed that the amplified DNA fragments of two Leishmania isolates(L.d.XJ771 and L.d.SC6) were all 392bp in length, point mutations were located in the two unique sequence (UQ-Ⅰ and UQ-Ⅱ),and no insertion/deletion found. The identities of comparison of Leishmania in GeneBank were more than 98%.Conclusion Sequence difference of the SSU rDNA variable region existed among Leishmania isolates from desert and hill foci; The sequences of the SSU rDNA variable region of L.d.XJ771 isolate and L. d.DD8 were identical.

关 键 词：利什曼原虫 小亚基核糖体核酸基因 聚合酶链反应 克隆 序列分析 基因变异 

分 类 号：R382.22[医药卫生—医学寄生虫学]