肿瘤患者血清细胞因子水平的变化  

Serum Cytokines Level Changes in Tumor -Bearing Hosts

作　　者：赵卫红[1] 施广霞[2] 袁小林[1] 钱振超[2] 

机构地区：[1]大连市第三人民医院,辽宁省大连市116037 [2]大连医科大学

出　　处：《中国肿瘤临床》2001年第3期176-178,共3页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金重点资助(39730440-Ⅱ)

摘　　要：目的：探讨肿瘤患者免疫偏斜状态及化疗时其的纠正作用。方法：采用ＥＬＩＳＡ法测定了肿瘤患者血清ＩＬ－２、ＩＬ－４、ＴＮＦ－α、ＩＦＮ－γ的水平及一线化疗后上述细胞因子的变化。结果：与正常人对照，ＩＦＮ－γ及ＴＮＦ－α呈显著下降，而 ＩＬ－２和ＩＬ－４变化不显著。正常人：ＩＦＮ－γ ２６６±１９３．５ｐｇ／ｍｌ，ＴＮＦ－α ２９１．２±６４．８ｐｇ／ｍｌ，ＩＬ－２ ３４６．２±１９８．２ｐｇ／ｍｌ，ＩＬ－４ １４８．９±４７．８ｐｇ／ｍｌ。带瘤组：ＩＦＮ－γ ４５．８±３１．２ｐｇ／ｍｌ，ＴＮＦ－α ６２．５±１４２ｐｇ／ｍｌ，ＩＬ－２ ５５２．３±７４２．５ｐｇ／ｍｌ，ＩＬ－４２４０．１±２２４．３ｐｇ／ｍｌ。术后组：ＩＦＮ－γ ３６．４±１８．８ｐｇ／ｍｌ，ＴＮＦ－α　３２．７±１５．１ｐｇ／ｍｌ，ＩＬ－２　２４５．１±１２３ｐｇ／ｍｌ，ＩＬ－４ １２１．６±１０７．５ｐｇ／ｍｌ。化疗后ＩＦＮ－γ和ＴＮＦ－α均有不同程度回升。带瘤组化疗前：ＩＦＮ－γ ３８．４±２９ｐｇ／ｍｌ，ＴＮＦ－α １８．１±１４ｐｇ／ｍｌ；化疗后：ＩＦＮ－γ ６２±５９．４ｐｇ／ｍｌ，ＴＮＦ－α ３５．４±２５．２ｐｇ／ｍｌ。术后组化疗前：ＩＦＮ?Objective To study the immune deviation in tumor patients and modulating effect of chemotherapy. Methods: In this experiment, the levels of serum IL-2, IL-4, TNF-a, IFN-γ in tumor patients and the changes of these cytokines after the first chemotherapy were measured with ELISA method. Results: As compared with normal control, the levels of serum IFN-γ and TNF-α in tumor patients decreased markedly, but the changes in IL-2 and IL-4 was not significant. Normal control: IFN-γ 266±193.5pg/ml, TNF-α 291.2±64.8pg/ml, IL-2 346.2± 198.2pg/ml, IL-4 148.9±47.8pg/ml. Tumor-bearing patients: IFN-γ 45.8±31.2pg/ml, TNF-α 62.5±142pg/ml, IL-2 552.3±742.5pg/ml, IL-4 240.1±224.3pg.ml. Patients after operation: IFN-γ 36.4±18.8pg/ml, TNF-α 32.7±15.1pg/ml, IL-2 245.1±123pg/ml, IL-4 121.6±107.5pg/ml. After routine chemotherapy, the levels of IFN-γ and TNF-α in tumor patients raised again in varying degrees. Tumor-bearing patients before chemotherapy: IFN-γ 38.4±29pg/ml, TNF-α 18.1±14pg/ ml. After chemotherapy: IFN-γ 62±59.4pg/ml, TNF-α 35.4±25.2pg/ml. Postoperative patients before chemotherapy: IFN-γ 45.8±57.2pg/ml, TNF-α 26.8±12.7pg/ml. After chemotherapy: IFN-γ 64.5±68.3pg/ml, TNF-α 32.9±15.9pg/ml. Conclusion: The modulation of cytokine network in tumor patients loses its balance and chemotherapeutic drugs play a modulating role on it.

关 键 词：细胞因子 免疫偏斜状态 化疗 肿瘤 ELISA 

分 类 号：R730.4[医药卫生—肿瘤]