聚合酶链反应分子灯塔法检测结核分枝杆菌  

PCR-molecular beacon assay for detecting Mycobacterium tuberculosis in clinical specimens

作　　者：李国利[1] 赵铭[1] 庄玉辉[1] 王巍[1] 夏湘萱[1] 徐淼[1] 戴海峰[1] 方翔[1] 

机构地区：[1]北京解放军第三○九医院结核病研究室,100091

出　　处：《中华检验医学杂志》2001年第2期82-83,共2页Chinese Journal of Laboratory Medicine

摘　　要：目的　评价聚合酶链反应 (PCR )分子灯塔法检测临床标本中结核分枝杆菌 (结核菌 )的应用价值。方法　应用涂片镜检法、培养法及PCR分子灯塔法检测 142份结核病患者痰标本 ,42份非结核呼吸系疾病患者痰标本。结果　涂片镜检法检测结核病患者临床标本中结核菌阳性率为 35 .2 % (5 0 / 142 ) ,培养法阳性率为 35 .9% (5 1/ 142 ) ,PCR分子灯塔法阳性率为 44 .4% (6 3/ 142 )。用PCR分子灯塔法检测临床标本特异性为 10 0 %。结论　PCR分子灯塔法是一种全新的PCR分子杂交模式 ,PCR扩增、荧光探针杂交、检测一体化 ,在单一管内完成 ,其简便、快速、防污染 ,敏感性较高、特异性强 。Objective To evaluate the value of PCR molecular beacon assay. In detecting mycobacterium tuberculosis in clinical specimens. Methods The sputum specimens of 142 patients with pulmonary tuberculosis and 42 patients with respiratory system diseases other than tuberculosis were tested by smears, cultures and PCR molecular beacon assays. Results The sensitivities of smears. cultures and molecular beacon assay in detecting mycobacterium tuberculosis in sputum specimens of patients with pulmonary tuberulosis were 35.2%,35.9% and 44. 4%, respectively. The specificity of molecular beacon assay in testing clinical specimens was 100%. Conclusion PCR molecular beacon assay is in a hermetially sealed simple tube, rapid and anticontaminative. This assay is higher in sensitivity and specifity,than smears and cultures is one of the effective mothods for diagnosis of tuberculosis.

关 键 词：结核分枝杆菌 聚合酶链反应 荧光探针杂交 分子灯塔法 

分 类 号：R446.5[医药卫生—诊断学]