常见肌酐测定方法中存在的干扰  

The interference of common methods in determination creatinine

作　　者：石凌波[1] 林龙顺[1] 

机构地区：[1]广州军区广州总医院检验科,510010

出　　处：《中华检验医学杂志》2001年第2期102-104,共3页Chinese Journal of Laboratory Medicine

摘　　要：目的　探讨目前常见肌酐测定方法中存在的干扰。方法　以漂白土 (fuller′searth)吸附法为标准 ,对Jaffe′氏反应速率法、紫外法、比色法和电极法进行方法学评价。结果　在所采用的 8种干扰物 (丙酮酸钠、α 酮戊二酸、胆红素、血红蛋白、肌酸、多巴胺、抗坏血酸、地塞米松 )中 ,Jaffe′氏反应速率法受干扰物影响最大 ,α 酮戊二酸 (≥ 0 0 6 15g/L)、抗坏血酸 (≥ 0 82mmol/L)、丙酮酸钠 (≥ 0 186mmol/L)对该法有正干扰 ,而胆红素 (≥ 16 5 5 μmol/L)则有明显负干扰 ;紫外法基本不受干扰物影响 ;比色法不仅受肌酸 (≥ 0 2 1mmol/L)的正干扰 ,而且也受多巴胺 (≥ 0 2 8mmol/L)的负干扰 ;电极法仅受到肌酸 (≥ 0 6 2mmol/L)的正干扰。Objective To investigate the interference of common methods in determination of creatinine. Methods Standardized with Fuller′s earth, Jaffe′s method, enzymatic method (ultraviolet), enzymatic method (colour), enzymatic method (electrode) were compared and evaluated. Results In eight interference materials, Jaffe′s method was positively affected by pyruvic acid (≥0 186 mmol/L), α ketoglutaric acid(≥0 0615 g/L), ascorbic acid(≥0 82 mmol/L) and negatively affected by bilirubin(≥165 5 μmol/L). Enzymatic method (ultraviolet) was not interfered. Enzymatic method (color) was positively affected by creatine(≥0 21 mmol/L) and negatively interfered by dopamine(≥0 28 mmol/L). At last, enzymatic method (electrode) was positively interfered by creatine(≥0 62 mmol/L). Conclusion Enzymatic method(ultraviolet) is the best in the above methods.

关 键 词：肌酐 漂白土 吸附法 酶法 紫外法 血清诊断 

分 类 号：R446.1[医药卫生—诊断学]