胃癌及胃癌前病变中E-cadherin及其相关蛋白的表达  

Expression of E-cadherin,α-catenin,β-catenin,andγ-catenin in Carcinoma and Precancerous Lesions of Stomach

作　　者：周绍弼[1] 滕小军[2] 陈新皓[1] 陈德基[3] 周福深 

机构地区：[1]湖北省黄石市第五医院病理科,湖北黄石435005 [2]湖北省黄石市大冶铁矿职工医院,湖北黄石435006 [3]武汉大学医学院病理教研室,湖北武汉430080

出　　处：《癌症》2001年第5期480-484,共5页Chinese Journal of Cancer

摘　　要：目的:探讨Ecadherin、αcatenin、βcatenin和γcatenin与胃癌发生及胃癌生物学行为的关系。方法:用免疫组织化学方法检测Ecadherin及αcatenin、βcatenin、γcatenin在43例胃癌,22例胃癌前病变及10例正常胃粘膜中的表达。结果:在胃癌前病变中Ecadherin,αcatenin、βcatenin、γcatenin的异常表达率分别为22.7%、22.7%、18.2%、27.3%,分别与胃癌Ecadherin(53.5%),αcatenin(55.8%)、βcatenin(51.2%)的异常表达率相比差异有显著性(P<0.05)。进展期胃癌异常表达率较早期胃癌高,除γcatenin外差异均有显著意义(P<0.05或P<0.01)。低分化胃癌Ecadherin的异常表达率较高,胃癌浸润到浆膜外及伴随淋巴结转移时Ecadherin、βcatenin的异常表达率升高(P<0.05或P<0.01)。76.7%的胃癌及50.0%的胃癌前病变Ecadherin系统中至少有一项表达异常,二者差异有显著性(P<0.05),且至少有一项为表达异常病人的淋巴结转移率明显高于无蛋白异常表达者(P<0.01)。结论:Ecadherin及部分相关蛋白的表达异常与胃癌的分化程度、临床分期、浸润深度及淋巴结转移密切相关,Ecadherin系统在由胃不典型增生转化为胃癌的过程中起作用,联合检测Ecadherin及αcatenin、βcatenin、γcatenin可提高检测结果的参考价值。Objective:The aim of this study was to evaluate t he expression of E-cadherin,α-catenin,β-catenin,andγ-catenin in gastric carcinoma and dysplasia and to determine the relatio nship with tumorigenesis and biolog ical behavior of gastric cancer.Methods :The expression of E-cadherin,α-catenin,β-catenin,andγ-catenin in 43patients with gastric carcinoma and gastric biopsy specim ens from 22patients with dysplasia a nd 10healthy controls were determin ed using immunohistochemistry.Results:Membranous staining was observed in control biopsy specimens for all com ponents of the complex.Abnormal expressive rates of E-cadherin,α-catenin,β-catenin in gastric carcinomas(53.5%,55.8%,51.2%,respectively)were significantly higher than that in gastric mucosal dysplasials(22.7%,22.7%,18.2%,respectively P<0.05),and the rates in advanced gastric ca rcinomas were also significantly hi gher than that in early gastric carcinoma (P<0.01,P<0.05,P<0.01,respectively).Tumors with a decrease in E-cadherin occurred significantly more frequ ently in undifferentiated gastric carcinom a(P<0.05).There were higher abnormal expressive rates of E-cadherin andβ-catenin in the patients with tumor infiltrating out of serosa and with lymph nodes metastasis(P<0.01or P<0.05).Up to 50.0%of gastric dysplasials a nd 76.7%of tumors stained abnormally for one or more components of the cadherin-catenin complex(P<0.05),and the lymph nodes metastasis rate s for one or more components of the E-cadherin complex was significantly higher than that for no one components of the E-ca dherin complex(P<0.01).Conclusion:The decreased expression of E-cadherin and part of the catenins correlate with tumor stage,poor differentiation,infil trative tumor growth,and lymph nodes metastasis,which suggests that E-cadherin complex play a critical role in the course of c hang from gastric mucosal dysplasia ls to gastric carcinoma.Thus,study of all the components of E-cadherin-catenin c omplex may be more valuable than single component of the complex for the detection of patie

关 键 词：胃肿瘤 E-钙粘附蛋白 连接蛋白类 免疫组织化学反应 癌前状态 

分 类 号：R735.2[医药卫生—肿瘤] R730.23[医药卫生—临床医学]