nm23-H1基因和增殖细胞核抗原在肝癌组织中的表达及其与癌细胞DNA含量的关系  

Relationship between nm23-H1, proliferating cell nuclear antigen expression and DNA content of human hepatocellular carcinoma

作　　者：赵新[1] 丛文铭[1] 谭璐[1] 吴孟超[2] 

机构地区：[1]第二军医大学东方肝胆外科医院病理科 [2]第二军医大学东方肝胆外科医院外科,上海200433

出　　处：《中华实验外科杂志》2001年第2期117-119,共3页Chinese Journal of Experimental Surgery

基　　金：上海市卫生系统百人计划资助项目 (98BR0 0 7)

摘　　要：目的　探讨转移抑制基因nm 2 3 H1在肝癌组织中的表达和癌细胞增殖及DNA含量间的关系。方法　应用免疫组织化学染色法检测 5 6例肝癌组织标本中nm 2 3 H1基因和增殖细胞核抗原 (PCNA)的表达 ,应用图像分析系统测定癌细胞DNA含量 ,分析与肝癌病理生物学行为之间的关系。结果　nm2 3 H 1表达阳性率无包膜组肝癌 (2 9.6% )比包膜完整 (64 .3 % )和包膜突破组肝癌 (66.7% )明显减低 (P <0 .0 5 )。DNA指数 (DI)与肝癌包膜情况、组织类型、组织分级也有显著相关性 (P <0 .0 5 )。nm 2 3 H1表达阴性的肝癌PCNA标记指数 (LI)高于nm 2 3 H1阳性者 (P <0 .0 5 ) ;PCNA标记指数高增殖组肝癌DI值 (2 .3 0± 0 .90 )较低增殖组肝癌DI值 (1.86± 0 .7)明显增高 (P <0 .0 5 )。结论　nm 2 3 H1表达与肝癌包膜形成具有一定关系 ,并与肝癌增殖活性相关。Objective To evaluate the relationship between expressions of nm23 H1, proliferating cell nuclear antigen (PCNA) in hepatocellular carcinoma and DNA content of cancer cells. Methods The expression of nm23 H1 and PCNA were detected in 56 cases of HCC by using immunohistochemistry technique and DNA content of cancer cells were analyzed by DNA imaging analyses system as well in order to find their relationship with biopathologic characters of HCC. Results The expression levels of nm23 H1 in HCC without encapsulation (29.6%) were significantly reduced when compared with that with encapsulation (64.3%) or with incomplete capsulation (66.7%, P <0.05). The increased DNA content of HCC was correlated with non encapsulation, compact type and high grade of the tumor ( P <0.05). The labeling indexes of PCNA in the group with negative nm23 H1 expression of HCC were significantly higher than those in the group with positive nm23 H1 expression of HCC ( P <0.05). The DI in high proliferating group of HCC(2.30±0.90) were significantly higher than in low proliferating group (1.86±0.70, P <0.05). Conclusion The expression of nm23 H1 in HCC was correlated with formation of tumor capsulation and cancer cell proliferating activity. The analysis of DNA content of tumor cells in combination with the immuohistochemistry staining of PCNA could give accurate index for the malignant biopathologic characters of HCC.

关 键 词：肝细胞癌 基因nm23-H1 基因表达 增殖细胞核抗原 免疫组织化学 DNA含量 

分 类 号：R735.7[医药卫生—肿瘤] R730.23[医药卫生—临床医学]