CpG-ODN可增强痘苗病毒修饰的癌溶物的抗肿瘤效果  

CpG-ODN Enhances Anti-Tumor Effects of Non-Replicating Vaccinia Virus-Modeified Oncolysates

作　　者：郭斐[1] 许洪林[1] 陆柔剑[1] 李军[1] 阮力[1] 

机构地区：[1]中国预防医学科学院病毒学研究所遗传免疫室,北京100052

出　　处：《中国肿瘤生物治疗杂志》2001年第1期6-9,共4页Chinese Journal of Cancer Biotherapy

基　　金：国家863高技术研究发展计划资助! (863 10 2 0 7 0 2 0 1);国家自然科学基金! (3 95 2 5 0 0 1;3 0 0 70 711)

摘　　要：目的 :研究CpG联合非复制重组痘苗病毒在肿瘤免疫治疗中的效果。方法 :构建非复制重组痘苗病毒v△ 11β75 ,联合应用免疫刺激寡核苷酸和重组痘苗病毒v△ 11β75进行肿瘤免疫治疗。 结果 :重组痘苗病毒v△ 11β75在人源 143细胞中的不能正常繁殖 ,Southern blot证实痘苗病毒天坛株HindⅢC ,K片段间基因的缺失。在Wistar大鼠的Walker′s肿瘤模型中 ,联合应用CpG ODN和非复制痘苗病毒修饰的WRC2 5 6细胞裂解物进行免疫治疗 ,可以延长荷瘤鼠的生存期 ,肿瘤生长速度降低。结论 :CpG ODN可以增强非复制痘苗病毒修饰的肿瘤细胞裂解物的抗肿瘤治疗效果 ,为肿瘤免疫治疗提供了一种新的途径。Objective:To investigate the antitumor effect of CpG-ODN combining non-replicating recombinant vaccinia virus in tumor immunotherapy.Methods: CpG-ODN and constructed vaccinia virus v△11β75 were combined to study the antitumor effects in the walker′s rat tumor model.Results: Recombinant vaccinia virus v△11β75 lost the replicating capacity on human cell line,143TK - cell.The genes between HindⅢ C & K fragment of vaccinia virus TianTan strain were deleted,verified with Southern-blot. In the Walker′s tumor model of Wistar rats,Combinational immunotherapy with CpG-ODN and non-replicating vaccinia virus-modified WRC256 oncolysates resulted in prolonged life span and reduced tumor hyperplasia. Conclusion: CpG-ODN can enhance the antitumor effects of non-replicating vaccinia virus-modified oncolysates,providing a new route of tumor therapy.

关 键 词：CPG-ODN 痘苗病毒 肿瘤 免疫治疗 

分 类 号：R730.51[医药卫生—肿瘤]