外阴癌DNA含量、癌基因产物rasP21检测及其临床意义的研究  

Detection and clinical evaluation of the DNA content and ras P 21 of the vulvar carcinoma

作　　者：吴强[1,2] 孙志华[1,2] 苏应宽[1,2] 刘新民 王家跃[1,2] 

机构地区：[1]徐州医学院附属医院 [2]山东省立医院

出　　处：《现代妇产科进展》1996年第4期312-314,共3页Progress in Obstetrics and Gynecology

摘　　要：目的：研究外阴鳞癌的ＤＮＡ含量、细胞增殖活力和ｒａｓ癌基因表达产物ｐ２１的变化及临床病理因素。方法：采用细胞免疫荧光染色技术和流式细胞术。结果：外阴鳞癌的ＤＮＡ含量、ＤＮＡ倍体、ＰＩＳ％、ＦＩ值随组织学分级的升级而增高：ＤＩＰＩＳ％值较高的肿瘤较易发生淋巴结转移；组织分化较差的肿瘤临床进展较快：二倍体肿瘤患者的预后好于异倍体肿瘤的患者。结论：应用流式细胞术检测外阴癌ＤＮＡ含量和ｒａｓＰ２１是一项有价值的临床研究。Objective To study the relationships among DNA content,cell proliferative rasP 21 expression and clinicopathological factors. Methods Flow cytometry and immunofluorescence staining technique were used. Results DNA content,DNA ploid,PI,S% and FI were increased with the rise of histopathological degree.Lymphatic matastases were more in that of neoplasms with higher DI,PI,S% values.Clinical progress was faster in that of neoplasms with higher histopathological degree.Patients with diploid tumors survived significantly longer than those with aneuploid tumors. Conclusions Detection of DNA content and rasP 21 of vulvar carcinoma by using flow cytometry and immunofluorescence staining technique is a valuable clinical research.

关 键 词：外阴肿瘤 脱氧核糖核酸 RAS癌基因产物 预后 流式血细胞计数 P21 DNA 

分 类 号：R737.35[医药卫生—肿瘤]