邻苯二甲酸二丁酯(DBP)诱导肿瘤细胞凋亡机制的初步研究  

THE PRELIMINARY STUDY ON THE MECHANISMS OF TUMOR CELLS APOPTOSIS INDUCED BY DIBUTYL PHTHALATE (DBP)

作　　者：张金红[1] 徐畅[1] 张凤川 张津辉[3] 侯奕[1] 朱毅宏[1] 吴祖泽[3] 

机构地区：[1]南开大学分子生物学研究所,天津300071 [2]山东省莒南县皮肤病防治站,山东莒南276600 [3]军事医学科学院放射研究所,北京100850

出　　处：《南开大学学报（自然科学版）》2001年第1期25-29,共5页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：国家自然科学基金重大项目 !(39730 0 55)

摘　　要：邻苯二甲酸二丁酯 (DBP)在体外对肺巨细胞癌细胞 (PG)和人早幼粒白血病细胞 (HL-60 )的增殖有明显的抑制作用 ;经 DBP处理过的 PG细胞 ,DNA断裂 ,在琼脂糖凝胶电泳上呈现出特异的断裂带 ;流式细胞术分析表明 ,DBP能提高 PG细胞的凋亡率 ,并且随作用时间的延长而增大 ;斑点杂交的结果显示 ,被 DBP处理过的 HL-60细胞中某些原癌基因、抑癌基因和肿瘤抑制基因的 m RNA表达水平都有所下降 ,这提示 DBP对Dibutyl phthalate (DBP) showed significant inhibitory effect on the proliferation of lung cancer cells (PG) and leukemic cells (HL 60) in vitro. With the exposure of PG cells to DBP, DNA degraded in certain way produced specific fragments on agarose gel electrophoresis. Flow cytometric analysis indicated that DBP enhanced the apoptotic rate of PG cells, which depended on the duration of interaction. The results of dot blotting demonstrated that the mRNA expression level of c H ras, c myc,Rb,p53 and bcl 2 in HL 60 cells treated by DBP decreased, which implicated that the suppression of DBP on the expression of the these oncogenes and mutant tumor suppressor genes may be one of the mechanisms by which DBP inhibiting the HL 60 cell proliferation.

关 键 词：邻苯二甲酸二丁酯 肿瘤细胞 细胞凋亡 诱导机制 增殖抑制 原癌基因 抑癌基因 

分 类 号：R730.21[医药卫生—肿瘤]