一氧化氮和氧自由基诱导视网膜神经节细胞凋亡  

Apoptosis in cultured rat retinal ganglion cells induced by nitric oxide and oxygen free radical

作　　者：张俊华[1] 张国安[2] 金威尔[1] 林颖[1] 

机构地区：[1]福建省人民医院眼科,福州市350004 [2]解放军第476医院临床免疫研究室

出　　处：《眼科新进展》2001年第2期84-86,共3页Recent Advances in Ophthalmology

摘　　要：目的　探讨一氧化氮 (NO)和氧自由基 (O- 2 )诱导培养大鼠视网膜神经节细胞 (RGCs)凋亡的作用及 2种作用因素间的关系。方法　体外培养大鼠 RGCs,用细胞形态学变化和 DNA断裂百分率来确定 NO供体 -硝普钠 (SNP)以及 O- 2 产生体系黄嘌呤氧化酶 (XO)和黄嘌呤 (X)所致的RGCs凋亡情况。结果　 (1) NO诱导组较对照组的细胞凋亡百分率和 DNA断裂百分率显著增高 ,且与 SNP的浓度相关 ,加入超氧化物歧化酶 (SOD)后可明显抑制 SNP所致的RGCs凋亡 ;(2 ) O- 2 损伤组的结果与 NO损伤组结果相似 ,同时应用 O- 2 和 NO组所致 RGCs的凋亡作用显著高于单独应用 O- 2 组或 NO组。结论　 NO和 O- 2 都能诱导培养的RGCs凋亡 。Objective To study the apoptosis in cultured rat retinal ganglion cells(RGCs) induced by nitric oxide (NO) and oxygen free radical (O - 2),and to explore the relationship between NO and O - 2.Methods The SNP (the provider of NO) and XO/X (provider of O - 2) were evaluated for their damage to RGCs. Damage was confirmed by cell apoptosis rate and percentage of DNA fragmentation.Results (1) Compared with control group ,cell apoptosis rate and percentage of DNA fragmention caused by SNP increased significantly ( P < 0.01) and was related to the concentration of SNP. The apoptosis in RGCs caused by SND can be inhibited evidently after oxygen free radical dismutase(SOD)was added.(2) The results of O - 2 damage to RGCs were similar to those of SNP.More serious damage was caused by using NO and O - 2 together than by using NO or O - 2 separately ( P < 0 01).Conclusion Both NO and O - 2 can induce the apoptosis in cultured RGCs,and there is cooperation between them.

关 键 词：一氧化氮 氧自由基 视网膜神经节细胞 凋亡细胞 

分 类 号：R774.1[医药卫生—眼科]