胃癌组织中p53和血管内皮生长因子的表达与血管生成的关系及其意义  

Correlation of p53 and vascular endothelial growth factor expression to angiogenesis in gastric carcinoma and its clinical significance

作　　者：明学志 尹浩然[2] 朱正纲[2] 燕敏[2] 薛建元[2] 王瑞年[3] 林言箴[2] 

机构地区：[1]江苏肿瘤防治研究所,南京210009 [2]上海第二医科大学附属瑞金医院 [3]上海第二医科大学病理教研室

出　　处：《中华普通外科杂志》2001年第3期141-144,共4页Chinese Journal of General Surgery

摘　　要：目的　探讨胃癌组织中p5 3与血管内皮生长因子 (VEGF)表达和血管生成的关系。方法　应用免疫组织化学技术对 6 0例胃癌组织进行p5 3、VEGF表达和肿瘤组织微血管密度MVD检测。结果　MVD以及p5 3和VEGF的表达与胃癌病灶的大小 (P <0 0 5 )、浸润深度 (P <0 0 1)、淋巴结转移 (P <0 0 1)和TNM分期 (P <0 0 1)密切相关。p5 3的表达与VEGF的表达显著相关 (χ2 =2 9 5 1,P <0 0 1) ;且p5 3和VEGF均阳性的胃癌组织MVD明显高于两者均阴性者 (P <0 0 1)。生存分析显示 :死亡组的平均MVD〔(5 0± 19)个 /视野〕显著高于存活者〔(2 7± 6 )个 /视野〕(P <0 0 1)。多元回归分析表明MVD是影响预后的独立因素。结论　胃癌组织突变的p5 3可上调VEGF的表达 ,促进血管生成 。Objective To investigate the relationship between the expression of p53 and vascular endothelial growth factor (VEGF) to angiogenesis in gastric carcinoma. Methods The expression of p53、VEGF and microvascular density (MVD) in gastric carcinoma was examined by immunohistochemical staining in 60 cases.Results There was a close correlation between MVD, p53 as well as VEGF expression and several clinicopathological factors including tumor size(P<005), penetrating depth(P<001), lymph node metastasis(P<001) and TNM stage(P<001). Increased expression of p53 and VEGF was significantly correlated with an increase in MVD(P<001).The average MVD in dead cases (50±19) was higher than that in the survivals (27±6); Multivariate regression analysis showed that MVD is an independent prognostic factor Conclusions These findings indicated that the mutant p53 may stimulate angiogenesis through VEGF upregulation in gastric carcinoma and accordingly influence tumor progression.

关 键 词：胃肿瘤 P53基因 内皮生长因子 新生血管化 病理性 

分 类 号：R735.2[医药卫生—肿瘤] R730.231[医药卫生—临床医学]