C-myc基因扩增、p16基因变异和乙型肝炎病毒DNA整合与肝细胞癌的相关性研究  

C-myc gene amplification, p16 gene alterations and HBV-DNA integration in hepatocellular carcinoma

作　　者：邱法波[1] 王培林[2] 吴力群[2] 张建立[2] 

机构地区：[1]青岛医学院附属医院普外科,266003 [2]青岛医学院生物教研室

出　　处：《中华普通外科杂志》2001年第3期155-157,共3页Chinese Journal of General Surgery

摘　　要：目的　探讨C myc基因扩增、p16基因变异和乙型肝炎病毒 (HBV)DNA整合与肝细胞癌 (HCC)的相关性。方法　应用差异聚合酶链反应 (d PCR)分析C myc基因扩增 ,用聚合酶链反应(PCR)和单链构象多态 (SSCP)分析p16基因变异 ,用PCR法检测整合型HBV DNA。结果　 (1)HCC组外周血、癌和癌旁肝组织中C myc基因扩增阳性率分别为 48% (14/ 2 9)、45 % (13/ 2 9)和 5 2 %(15 / 2 9) ,3者间差异无显著意义 (χ2 =0 0 0 0 8,P >0 0 5 ,自由度υ =2 ) ,但明显高于肝硬化 (LC)组外周血和LC组织阳性率〔0 (0 / 12 )和 8% (1/ 12 ) ,P均小于 0 0 5〕。 (2 ) 2 9例癌组织中有 3例p16基因纯合性缺失 ,未发现点突变。 (3)正常肝、肝硬化和肝癌组织中整合型HBV DNA阳性率分别为 14%(2 / 14)、6 7% (8/ 12 )和 97% (2 8/ 2 9) ,3者间差异有显著意义 (χ2 =2 9 434 5 ,P <0 0 1)。结论　 (1)HCC的发生与C myc基因扩增密切相关 ;(2 )HCC中p16基因的纯合性缺失发生频率为 10 % ;(3)HBV DNA整合与HCC的发生密切相关 ;(4 )对外周血的检测能反映肝细胞C myc基因扩增和HBV DNA整合情况。Objective To observe the roles of C-myc gene amplification, p16 gene alterations and HBV-DNA integration in the pathogenesis and progression of hepatocellular carcinoma(HCC). Methods Differential polymerase chain reaction (d-PCR) was used for defining the amplification of C-myc oncogene Alterations of exon 1 and 2 in p16 gene were analysed by PCR and single-strand conformation polymorphism (SSCP) silver staining method HBV-DNA integration was assayed by PCR Results (1)The positive rates of C-myc gene amplification of peripheral blood (PB),tumor and their adjacent liver tissues in HCC group (29 cases) were 48%(14/29), 45%(13/29) and 52%(15/29), respectively The discrepancies among them were not statistically significant, (χ2=0000*!8, P>005,υ=2), but they were significantly higher than that of PB(0%, 0/12) as well as liver cirrhosis (LC) tissues (8%, 1/12) in LC group (12 cases), respectively (2)A total of 3 homozygous deletions and no point mutations of exon 1 and 2 in p16 gene in HCC were found (3)The discrepancies of the positive rates of HBV- DNA integration among normal liver (14%, 2/14), LC(67%, 8/12) and HCC (97%, 28/29) were statistically significant (χ2=29434*!5, P<001,υ=2) Conclusions (1)C-myc gene amplifications were closely related to hepatocarcinogenesis (2)The homozygous deletions and point mutations of p16 gene were infrequently encountered in HCC (3)HBV-DNA integration was closely ralated to the progression in HCC (4)The tests of PB indicates the C-myc gene amplification and HBV-DNA integration in hepatic cells

关 键 词：肝细胞癌 癌基因 C-MYC基因 P16基因 HBV-DNA 基因扩增 

分 类 号：R735.7[医药卫生—肿瘤]