基因重组鼠伤寒沙门氏菌防龋疫苗的研究  

Recombinant salmonella typhimurium anticaries vaccine Ⅲ. Identification of recombinant expressing plasmid carrying streptococcus mutans pac gene A region

作　　者：陈罕[1] 凌均启[1] 林正梅[1] 杨国平[1] 涂家珍[1] 高燕[1] 

机构地区：[1]中山医科大学口腔医学院,广东广州510080

出　　处：《牙体牙髓牙周病学杂志》2001年第1期5-6,共2页Chinese Journal of Conservative Dentistry

基　　金：广东省自然科学基金资助课题!(970 10 5 )

摘　　要：目的 :确认变形链球菌表面蛋白pac基因A区片段重组表达质粒载体pET - 17b -A中的A区片段阅读框正确。方法 :利用ABI 310DNA自动测序仪进行DNA序列测定 ,并利用Internet进行联机检索 ,确定序列的构建正确与否。结果 :测定了重组表达质粒载体pET - 17b -A中的 35 0个碱基序列 ,联机检索序列的构建正确。结论 :重组表达质粒载体pET - 17bAIM: To confirm and correct the open reading frame of pac gene's A region in pET-17b-A. METHODS: pET-17b-A was sequenced by autosequence instrument ABI1310. The sequence was matched to the GenBank via Internet. RESULTS: The sequenced 350 bases matched the pac gene's A region after they were searched in GenBank. CONCLUSION: The open reading frame of pET-17b-A was correct.

关 键 词：变形链球菌 pac基因 阅读框 防龋疫苗 

分 类 号：R780.2[医药卫生—口腔医学]