人胃腺癌长春新碱耐药细胞MKN28/VCR和MKN45/VCR的建立  

Establishment and characterization of vincristine-resistant MKN28/VCR, MKN45/VCR of human gastric cancer cell lines

作　　者：俞丽芬[1] 章永平[1] 乔敏敏[1] 吴云林[1] 

机构地区：[1]上海第二医科大学瑞金医院消化科,上海市200025

出　　处：《世界华人消化杂志》2001年第3期297-301,共5页World Chinese Journal of Digestology

基　　金：上海市教育委员会基金;No.96B11

摘　　要：目的为研究消化道肿瘤的耐药机制,建立了2株体外人胃腺癌长春新碱耐药细胞系MKN28/VCR和MKN45/VCR。方法应用电镜、流式细胞仪观察细胞超微结构和细胞周期分布的变化:MTT法进行耐药倍数、交叉耐药和逆转耐药的实验研究。结果 MKN28/VCR,MKN45/VCR的细胞超微结构和细胞周期分布与亲本细胞有显著差异。对长春新碱耐药倍数MKN28/VCR为7.1倍,MKN45/VCR为5.4倍,并与阿霉素、顺铂、丝裂霉素、5 氟尿嘧啶、依托泊甙存在交叉耐药现象:丁基硫堇亚胺和维拉帕米都有逆转耐药的作用。结论 MKN28/VCR和MKN45/VCR是比较符合消化道肿瘤特点的长春新碱耐药细胞系。AIM To study drug resistance mechanism of gastrointestinal tumor, we developed MKN28/VCR, MKN45/VCR which were vincristine-resistant cell lines of human gastric cancer. METHODS The transmission and scanning electron microscopy and flow cytometry were used to observe the changes of cells ultra-structure and cell cycle distributions. The drug resistance, cross-resistance and reversing drug resistance were studied by MTT. RESULTS The cells ultra-structure and cell cycle distributions of MKN28/ VCR and MKN45/ VCR were different from their parent cells. MKN28/ VCR was 7.1 times and MKN45/VCR was 5.4 times more resistant to cytotoxic action of vincristine. Two resistant sublines were cross-resistant to ADM, CDDP, MMC, 5-Fu, and Vp16. Buthionine sulphoximine (BSO)and verapamil (VPM) could reverse the multi-drug resistance of vincristine resistant cell lines. CONCLUSION Vincristine-resistant cell lines MKN28/VCR and MKN45/VCR has many characteristics of gastrointestinal tumor.

关 键 词：胃肿瘤 长春新碱 丁基硫堇亚胺 维拉帕米 药物耐受性 细胞系 治疗 

分 类 号：R735.2[医药卫生—肿瘤]