体外培养大鼠星形细胞的缺糖缺氧性损伤及药物的保护  

OXYGENANDGLUCOSE DEPRIVATION-INDUCED INJURY IN CULTURED RAT ASTROCYTES AND THE PROTECTIVE EFFECTS OF DRUGS

作　　者：董丽萍[1] 王天佑[1] 

机构地区：[1]北京市神经外科研究所

出　　处：《中国应用生理学杂志》1996年第2期107-110,共4页Chinese Journal of Applied Physiology

摘　　要：本实验用大鼠星形细胞体外培养模型，观察了细胞在不同条件下乳酸脱氨酶（ＬＤＨ）的漏出。发现当去掉葡萄糖后，细胞缺氧５和７ｈ后ＬＤＨ漏出明显增加，５ｂ为５９．７±２５．３Ｕ／ｍｇ蛋白（对照组２４．７±１６．３，Ｐ＜０．０５），７ｈ为６８．３±８９Ｕ／ｍｇ蛋白（对照组３９．９±２１２，Ｐ＜０．０１）。用３－氧－甲基－［１－￣３Ｈ］－Ｄ－葡萄糖摄取法测量细胞体积，结果显示缺糖缺氧５ｈ后细胞明显肿胀，从对照组的４．１±１．２增加到８．１±３．２μｌ／ｍｇ蛋白（Ｐ＜０．０１）。丹参有效成分７６４－３在０．５至５０μｍｏｌ／Ｌ时能减少缺糖缺氧细胞ＬＤＨ漏出；在５０μｍｏｌ／Ｌ时能减小缺糖缺氧细胞的体积。谷氨酸受体拮抗剂ＤＮＱＸ（６，７－ｄｉｎｉｔｒｏｑｕｉｎｏｘａｌｉｎｅ－２，３ｄｉｏｎｅ）５０μｍｏｌ／Ｌ能减轻星形细胞肿胀和ＬＤＨ漏出。The effects of severe O_2 and glucose deprivation were studied in a subculture of astrocytesprepared from neonatal rat cerebral cortices.After two weeks of incubation in vitro,culturedastrocytes were further incubated with Earle's solution and subjected to hypoxic condition at95% N_2+5% CO_2 for3,,7h.When glucose was taken out from the culture medium,significantcell damage occurted at 5 and 7h of hypoxia,as evidenced by a time,dependent leakage of lactatedehydrogenase(LDH), being 59.7+ 25.3U/mg protein at 5h (24.7± 16.3 in control,p<0.05)and 68.3 ± 8.9 U/mg protein at 7h(39.9 ± 21.2 in control,P<0.01),The cell volume ofastrocytes was measured with 3-O-methyl-[1-3H]-D-glucose uptake. The volume of astrocytesincubated for 5h without glucose and oxygen increased remarkably,from 4.1±1.2μl/mg proteinin control group to 8.1 ± 3.2 μl/mg protein(P<0.01).Under this condition 764-3.an activecomponent of Chinese medicinal herb Salvia mIjliorrhiza Bge,(50μmol/L) or 6,7-dinitroquinoxalin-2,3-dione(DNQX),an antagonist to AMPA/KA subtype of glutamatereceptors,(50μmol/L)significantly decreased cell swelling and the leakage of LDH.

关 键 词：星形细胞 缺氧 乳酸脱氢酶 细胞体积 丹参 DNQX 脑水肿 药物保护 

分 类 号：R742.702[医药卫生—神经病学与精神病学] R971[医药卫生—临床医学]