人胎肝中低分子抑瘤物的分离纯化、结构鉴定及其对肿瘤细胞生长的选择性抑制作用  

ISOLATION,PURIFICATION AND STRUCTURE ELUCIDATION OF A LOW MOLECULAR WEIGHT TUMOR SUPPRESSOR FROMHUMAN FETAL LIVER ANDITS SELECTIVE SUPPRESSION ON TUMOR CELL GROWTH

作　　者：张庆林[1] 吴祖泽[1] 曹菊荣[1] 薛惠华 裴雪涛[1] 王立生[1] 徐黎[1] 杜泽涵 冯锐 

机构地区：[1]北京放射医学研究所

出　　处：《中国应用生理学杂志》1996年第2期120-123,共4页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金

摘　　要：在胎儿组织中存在一类低分子肿瘤抑制物。胎肝细胞的甲醇－丙酮提取物中保留有大部分抑瘤活性。用体外液体培养条件下对人急性粒系白血病细胞系（ＨＬ－６０）的抑制作用为指标，跟踪指导分离过程。提取物经反相Ｃ１８中压液相色谱、ＳｅｐｈａｄｅｘＬＨ－２０凝胶色谱及氨基键合相高效液相色谱分离得到一纯活性物质，经ＮＭＲ和ＭＳ鉴定为７－酮基胆固醇（７－ｋｅｔｏｃｂｌｅｓｔｅｒｏｌ，７－ＫＣ）。体外琼脂培养条件下７－ＫＣ对小鼠ＷＥＨ１－３和Ｓ－１８０细胞较对正常小鼠骨髓粒一巨噬系祖细胞有更强的抑制增殖及集落生成作用。７－ＫＣ对ＨＬ－６０细胞增殖较对正常人骨髓ＣＦＵ－ＧＭ有更强的抑制作用。Our previous work has shown the existence of a low molecular weight tumor suppressor inhuman fetal tissue In the present workone suppressor was isolated and purified from methanolextract of human fetal liver,with the isolating process guided by suppression of HL-60 cellgrowth in vitro. The procedure for puriflcation included C 1 8 reverse-phase medium pressurechromatography,gel fiItration on Sephadex LH-20,and high-performance liquidchromatography on NH_2-bonded phase.The structure of the suppressor was elucidated to be7-ketocholesterol.Under the condition of in vitro agar plate culture,7-ketocholesterol showedpreferential inhibitory effects on the growth of both human and murine leukemic cell lines in-cluding HL-60,WEHI-3and murine S-180 cells but weaker effects on the growth of normalhuman and murine bone marrow granulocyte-macrophage progenitors(CFU-GM)

关 键 词：胎儿肝脏 低分子抑瘤物 分离 结构鉴定 7-酮基胆固醇 肿瘤细胞 选择性抑制 

分 类 号：R73－36[医药卫生—肿瘤]