共轭亚油酸对B16-MB细胞系粘附和运动能力的影响  

The effects of conjugated linoleic acid on the adherence and migration of B16-MB mouse melanoma cells

作　　者：陈丙卿[1] 薛英本[1] 丰维加 郑玉梅[1] 刘瑞海 

机构地区：[1]哈尔滨医科大学公共卫生学院,150001 [2]哈尔滨市南岗区卫生防疫站 [3]Cornell University,Ithaca

出　　处：《卫生毒理学杂志》2001年第1期20-22,共3页Journal of Health Toxicology

基　　金：国家自然科学基金资助!( 19870 661)

摘　　要：目的　观察共轭亚油酸 (conjugatedlinoleicacid ,CLA)对黑色素瘤细胞B16 MB黏附和运动能力的影响 ,探讨CLA预防肿瘤转移的可能作用途径。方法　采用鼠黑色素瘤B16 MB高转移细胞系 ,用 0、2 5、5 0、10 0、2 0 0μmol LCLA处理后 ,分别用黏附试验、随机运动试验、Transwell趋化运动模型来研究CLA对细胞的黏附和运动能力的影响。结果　经CLA处理后 ,B16 MB向细胞外基质成分的黏附能力降低 ,同时随机运动能力降低 ,但趋向运动能力未发现变化。结论　CLA可能通过抑制肿瘤细胞的粘附和运动能力来发挥预防肿瘤转移的作用。Objective To investigate the effects of conjugated linoleic acid on the adherence and migration of B16\|MB cells,and explore the possible pathway of the chemopreventive/preventive effects of conjugated linoleic acid on tumor metastasis.Methods B16\|MB cells were cultured in RPMI 1640 medium,10% fetal bovine serum and 0(controll),25,50,100,200 μmol/L CLA for 24hours and the ability of CLA\|treated cells to adhere to intracellular matrix and to move was investigated.Results The ability of CLA\|treated cells to adhere to fibronectin and laminin decreased significantly ( P<0.01 ).CLA inhibited the migration of B16\|MB,but no effect on the ability of B16\|MB tendency\|motion.Conclusion CLA may prevent metastasizing by affecting the adherence and migration ability of tumor cells.

关 键 词：其轭亚油酸 鼠黑色素瘤细胞 粘附 肿瘤转移 

分 类 号：R73－37[医药卫生—肿瘤]