人胃癌组织中生长抑素2型受体表达的初步研究  

A preliminary study on SSTR2 expression of human stomach cancer tissues

作　　者：迟志宏[1] 郑文尧[2] 梁浩[2] 孙晓华[2] 

机构地区：[1]北京军区总医院肿瘤科,北京100700 [2]解放军总医院消化科

出　　处：《中国肿瘤临床与康复》2001年第2期18-20,共3页Chinese Journal of Clinical Oncology and Rehabilitation

摘　　要：目的　研究胃癌组织中生长抑素 2型受体 (SSTR2 )的表达情况。方法　取一部分胃镜活检或手术组织标本 ,提取组织总RNA后 ,进行RT PCR ,以beta actin为内参照 ,经计算机图像分析系统测定SSTR2的数值 ,通过其mR NA的测定间接反映组织SSTR2的表达 ;另一部分组织标本送病理检查。结果　胃癌及萎缩性胃炎组织的SSTR2表达明显低于对照组 ,胃癌组织的SSTR2表达明显低于癌旁组织。不同部位胃癌的SSTR2表达无明显变化。分化程度较好的胃癌组织SSTR2的表达略高于分化程度差的胃癌组织 ,但无显著差异。结论　癌前状态 -萎缩性胃炎和胃癌组织中SSTR2明显降低 ,提示组织SSTR2降低可能是致癌因素之一 。Objective To measure the expression of SSTR2 in stomach cancer tissues.Methods The tissues from endoscopic biopsy and surgery were collected.After total RNA extraction,RT PCR technique was used to detect SSTR2 expression.In reference to the beta actin,the expression degree of SSTR2 was measured by a comuter picture analysis system;Meanwhile the tissues were examined pathologically.Results The expression of SSTR2 in the chronic atrophic gastritis and stomach tissues were significantly lower than that in the control group(P=0.0204).The expression of SSTR2 in the stomach cancer tissues was also significantly lower than that in the mucosa adjactent to cancer (P=0.045).In the stomach cancer group,the expression of SSTR2 was not significantly different in different sites,which was slightly higher in the better differentiated adenocarcinoma,without significant difference.Conclusions In the mucosa of chronic atrophic gastritis and stomach cancer,the expression of SSTR2 was significantly less seen,suggesting that the decrease of SSTR2 in tissues may be one of the carcinogenetic factors .The expression of SSTR2 in better differentiated stomach cancer was higher than that in poorly differentiated stomach cancer.

关 键 词：胃癌 生长抑素 SSTP2 病理 

分 类 号：R735.2[医药卫生—肿瘤]