McAb共刺激PBLs膜表面分子表达与肝癌细胞凋亡的相关性  

Correlation of McAb costimulation PBLs membrane surface molecular expression and hepatoma cell apoptosis

作　　者：明利华 陈维春[2] 林月霞[2] 黄树林[2] 符生苗[3] 邓立群[3] 

机构地区：[1]中国医学院学院肿瘤研究所,博士生北京100021 [2]广东药学院分子生物学研究室,广州510224 [3]海南省人民医院

出　　处：《中国免疫学杂志》2001年第4期195-197,共3页Chinese Journal of Immunology

基　　金：国家自然科学基金资助

摘　　要：目的 :研究McAb共刺激PBLs前后的表型变化及与肝癌细胞凋亡的相关性。方法 :采用流式细胞仪分析PBLs被刺激前后的表型 ,用电镜超微结构和DNA梯子电泳观察肝癌细胞凋亡。结果 :CD3、CD4分子表达高于刺激前约 10 % ,肝癌细胞出现凋亡 ,细胞核固缩、边聚、裂解。同时也出现典型的DNA梯子。而肝癌患者用共刺激PBLs后 ,CD3和CD8增高 (P <0 0 5 )。结论 :共刺激使共刺激信号分子增加 ,抗原呈递能力增强 ,也使细胞因子分泌增加 ,这些因素促使肝癌细胞凋亡 ,而肿瘤患者T细胞增加时向CD8+ 分化 ,结果提示膜分子的表达与凋亡密切相关。Objective:To study phenotype changes in PBLs costimulated by McAb and correlation of phenotype changes and apoptosis in hepatoma cell.Methods:Cell phenotype changes in PBLs were analyzed by flow cytometry.By means of hypermicroscopic structure and DNA ladder electrophoresis,hepatoma cell apoptosis was studied.Results:Expression of CD3、CD4 in stimulated PBLs was about 10 percent more than those without stimulation Nucleus pyknotic、border gathering and collapse were found in hepatoma cell,which showed typical apoptosis.DNA ladder appeared in electrophoresis of hepatoma DNA.CD3 and CD8 increased in tumor patients PBLs being co stimulated (P<0.05).Conclusion:Co stimulating signal molecular increased,presenting antigen enhanced and cytokine secreted more by co stumulating.Hepatoma cell apoptosis was bringed by these factors.T cells of tumor patients differentiated to CD8 +.Correlation of membrane molecular expression and apoptosis was showed by the results.

关 键 词：单抗共刺激 肝癌细胞 细胞凋亡 McAb PBLS膜表达分子 

分 类 号：R735.7[医药卫生—肿瘤]