CK13基因与EB病毒DNA在鼻咽癌中的表达及临床意义  

Cytokeratin 13 gene expression and Epstein - Bars virus infection in human nasopharyngeal carcinoma

作　　者：邱元正[1] 文忠[2] 林功标[1] 肖健云[1] 田勇泉[1] 赵素萍[1] 陶正德[1] 李桂源[3] 

机构地区：[1]中南大学湘雅医院耳鼻咽喉科,湖南长沙410008 [2]第一军医大学珠江医院耳鼻咽喉科,广东广州510282 [3]中南大学湘雅医学院肿瘤研究所,湖南长沙410078

出　　处：《中国耳鼻咽喉颅底外科杂志》2001年第1期18-20,共3页Chinese Journal of Otorhinolaryngology-skull Base Surgery

基　　金：国家863和973高技术计划经费资助项目!(102-10-01-05);湖南省自然科学基金资助项目!(99JJY20034)

摘　　要：目的探讨细胞角蛋白基因１３ （Ｃ １３）和ＥＢ病毒ＤＮＡ在人鼻咽癌（ＮＰＣ）组织中的表达及其意义。方法应用 Ｎｏｒｔｈｅｒｎ杂交对３２例 ＮＰＣ和 ８例慢性鼻咽炎（ＣＩＮＥ）组织进行ＣＫ１３ｍＲＮＡ水平检测，同时应用ＰＣＲ方法进行ＥＢ病毒ＤＮＡ检测。结果ＣＫ１３基因在鼻咽炎组织中高表达，在ＮＰＣ组织中的表达显著下调，下调的频率达６５．６３％（２１／３２）。结论ＮＰＣ组织的分化可能与ＣＫ１３基因的表达下调有关。Objective To investigate the possible pathogenetic role of the Cytokeratin 13 (CK13) gene expression and Epstein - Barr virus (ESV) infection in human nasopharyngeal carcinoma (NPC) and their clinical implications. Methods Tissue samples taken from a total of 32 cases of NPC and 8 cases of chronic inflammation of nasopharyngeal epithelium (CINE) were studied for the CK13 gene at the mRNA level by northern blot hybridization. Polymerase chain reaction (PCR) was used to detect EBV-DNA. Results A high expression of the CK13 gene was found in 100% (84) of CINE, and a low and negative expression of the gene in 65. 6% (21/32) of NPC.Conclusion Most patients of NPC are poorly differentiated. This is possibly related to the down-regulation of CK13 gene expression.

关 键 词：鼻咽癌 细胞角蛋白基因13 EB病毒 DNA 聚合酶链反应 NORTHERN杂交 

分 类 号：R739.63[医药卫生—肿瘤]