交链孢酚诱发人胎食管上皮增生的实验研究  

Research on the Epithelial Hyperplasia of Human Fetal Esophagus Induced by Alternariol (AOH)

作　　者：董伟华[1] 郑智敏[1] 刘桂亭[1] 钱玉珍[1] 杨红艳[1] 宫亚欧[1] 陈振光[2] 马长路[2] 

机构地区：[1]河南医科大学病理生理学教研室 [2]河南医科大学病理解剖学教研室

出　　处：《河南肿瘤学杂志》1991年第1期20-22,65,共3页Henan Journal of Oncology

摘　　要：应用AOH作用体外培养的人胎儿食管上皮组织24h,继续培养一周后,四例胎儿的食管上皮组织均表现基底层细胞增厚,达7～8层,是空白对照的2～3倍,细胞排列紧密紊乱,胞核增大,不规则且浓染,可见双核仁,并呈乳头状内生性增生,在固有层及粘膜下层形成上皮细胞团。用甲基硝基亚硝基胍(MNNG)处理人胎食管上皮,上皮组织亦增生;而溶剂对照组无明显改变。本实验结果提示AOH对人胎儿食管上皮具诱变作用,为AOH与人食管癌的关系提供了客观依据。Epithelial tissues of human fetal esophagus cultured in vitro were treated by Alternariol(AOH) for 24 hrs.and continued to be cultured for 7 days. The layer of the basal calls of the esophageal epithelial tissue was increased to 7 to 8 layers in all the four cases of human fetal, which is 2 to 3 times of the blank control group. The basal cells of the epithelial tissue were arranged tightly and disorderly, their nuclei were enlarged, irregular and stained heavily and the double nucleoli could be seen. The epithelial cells grew towards the lamina propria and submucosa, inverted growth was the character of the epithelial hyperplasia. The epithelial tissues were also hyperplastic in the positive control group in which the cultured esophageal epithelial tissues were treated by MNNG. In the blank and solvent control groups, the coltured epithelial tissues of the four cases of human fetal had no change. These results indicate that AOH possesses mutagenic effect on the esophageal epithelium of human fetal, and give the support to the suggest that there is some relationship between AOH and human esophageal cancer.

关 键 词：食管癌 交链孢酚 器官培养 

分 类 号：R735.102[医药卫生—肿瘤]