非经典HLA Ⅰ类分子(HLA-G和HLA-E)在人肿瘤细胞系的表达及IFN-γ的调节作用  

The Expression of Non－Classical HLA Class Ⅰ Molecules (HLA－G and HLA－E) in Human Tumor Cell Lines and the Regulatory Effect of IFN－γ

作　　者：张彩[1] 田志刚[1] 魏海明[1] 冯进波[1] 许晓群[1] 张建华[1] 孙汭[1] 

机构地区：[1]山东省医学科学院基础医学研究所 山东肿瘤生物治疗研究中心,济南市250062

出　　处：《中国肿瘤临床》2001年第4期247-250,253,共5页Chinese Journal of Clinical Oncology

基　　金：国家自然科学基金!(编号 39870729和39970828);山东省自然科学基金资助!(编号 Q99C07)

摘　　要：目的：探讨非经典HLA Ⅰ类分子在人肿瘤细胞系的表达及IFN－γ的调节作用。方法：用RT－PCR法检测12种肿瘤细胞系和人脑胶质瘤组织及妊娠妇女滋养层组织标本HLA－G和HLA－E mRNA的表达。结果：人脑胶质瘤组织及妊娠妇女滋养层组织均有HLA－G和HLA－E mRNA的表达；12株肿瘤细胞中仅人T细胞淋巴瘤Karpas存在HLA－G3的mRNA表达，经IFN－γ处理后，Karpas出现HLA－G1／G5和HLA－G2／G4的表达，宫颈癌Hela细胞、黑色素瘤M21细胞和膀胱癌T24细胞出现HLA－G3 mRNA的表达；12株肿瘤细胞中有9株表达HLA－E mRNA。结论：肿瘤存在HLA－G和HLA－E mRNA的表达，IFN－γ可促进HLA－G mRNA的表达。肿瘤细胞HLA－G和HLA－E的表达可能是肿瘤逃避免疫系统监视的机制之一。Objective: To explore the expression of nonclassical HLA class I molecules (HLA－G and HLA－E) in human tumor cells and the regulatory effect of IFN－γ． Methods: The HLA－G and HLA－E mRNA transcripts were detected by RT－PCR in 12 tumor cell lines, human glioma biopsies and first trimester trophoblast． Results: Human glioma biopsies and first trimester trophoblast exhibited HLA－G1／G5, G2／G4 and G3 mRNA transcripts． Among 12 tumor cells, only Karpas cells exhibited HLA－G3 mRNA transcripts． After treated with IFN－γ 1000u／ml for 48h, the HLA－G mRNA transcripts were increased significantly in Karpas, M21, Hela and T24 cells． 9／12 tumor cell lines exhibited HLA－G mRNA transcripts． Conclusion: HLA－G and HLA－E mRNA can be expressed in tumor cells． IFN－γ can promote the expression of HLA－G． The transcripts and expression of HLA－G and HLA－E may be a way for tumor cells to escape from immunosurveillance．

关 键 词：HLA-G HLA-E RT-PCR IFN-Γ 肿瘤 肿瘤急度逃逸 

分 类 号：R730.2[医药卫生—肿瘤]