白血病病人异基因外周血干细胞移植后淋巴细胞TCRVβ基因片段的选择性扩增  

Restricted expansion of T cell receptor (TCR)Vβ genes in leukemic patients subjected to allogeneic hematopoeitic stem cell transplantation

作　　者：杜欣[1] 李扬秋[2] 苏建华[1] 李树浓[2] 林伟[1] 黄梓伦[1] 

机构地区：[1]广东省人民医院血液科,广东广州510080510632 [2]暨南大学血液研究室,广东广州510632

出　　处：《中国病理生理杂志》2001年第4期305-307,共3页Chinese Journal of Pathophysiology

基　　金：广东省科委重点攻关项目! (No .199817) ;广东省卫生厅医学科研基金!资助 (No.A19980 17)

摘　　要：目的 :了解白血病病人异基因外周血干细胞移植后淋巴细胞TCRVβ基因片段选择性扩增的情况。方法 :采用RT -PCR扩增 5例CML和 2例AML移植后患者外周血的单个核细胞的TCRVβ 2 4个基因片段 ,分析其TCRVβ基因的表达情况。 结果 :不同标本中仅检测到 2 - 8个VβT细胞基因片段 ,不同患者中缺乏的TCRVβ基因片段不尽相同。结论 :移植后病人外周血淋巴细胞TCRVβ基因片段部分受抑制 。AIM: To investigate restricted expansion of TCR Vβ gene repertoire in patients with leukemia following allogeneic hematopoietic stem cell transplantation. METHODS: TCR Vβ subfamily genes in peripheral blood mononuclear cells from 7 cases of leukemia was amplified using RT-PCR. RESULTS: Only two-eight fragments of Vβ genes were detected in samples from these patients, and the detected fragments are different in different patients. CONCLUSION: TCR complexes were abnormal in all patients, part of the genes were seletively expansed and part of them were suppressed after transplantation.

关 键 词：异基因 白血病 造血干细胞 聚合酶链反应 TCR-Vβ基因 基因扩增 

分 类 号：R733.7[医药卫生—肿瘤]