流动注射化学发光测定甲基对硫磷  

Determination of Methyl-parathion Using Luminol-based Chemiluminescence Flow Injection Method

作　　者：饶志明[1] 王建宁[1] 李隆弟[1] 张新荣[1] 

机构地区：[1]清华大学化学系,北京100084

出　　处：《分析化学》2001年第4期373-377,共5页Chinese Journal of Analytical Chemistry

摘　　要：首次研究了农药甲基对硫磷在碱性介质中（ｐＨ：１１．５～１２．０）与鲁米诺和过氧化氢产生化学发光的行为及反应机理，并发现水溶性高分子聚乙二醇对该反应具有显著的增效作用，据此建立了流动注射化学发光测定甲基对硫磷的新方法。甲基对硫磷的浓度在 ５×１０－８～１．０ ×１０－５ｇ／ｍＬ范围内与化学发光强度呈良好的线性关系；检出限为 ２×１０－８ｇ／ｍＬ；对 １．０×１０－６ｇ／ｍＬ甲基对硫磷进行了１１次平行测定，相对标准偏差小于４％；标准加入回收率为８３％～９４％。该法应用于谷物中农药残余量的测定，结果满意。A simple, fast flow injection chemiluminescence (CL) method for the determination of methyl-parathion has been developed. It is based on the reaction of methyl parathion with luminol-H2O2 in the alkaline medium (pH: 11 .5-12.0), sensitized by water-soluble macromolecule Polyethylene glycol 400. Under the optimal conditions, the CL intensity was linear to the concentration in the range of 5 .0 × 10-8 -1 .0 ×10-5 g/mL (r = 0.9996), with a detection limit (3σ) of 2. 0 ×10-8 g/mL. Relative standard deviation was less than 4% (n = 11) and the recovery was between 82% - 93%. This method has been successfully applied to the determination of trace residue of methyl-parathion in grain sample.

关 键 词：化学发光 流动注射 甲基对硫磷 聚乙二醇-400 有机磷杀虫剂 谷物 农药残留量测定 

分 类 号：O657.3[理学—分析化学] TQ450.7[理学—化学]