IFN-γ抑制血管成形术后兔血管平滑肌细胞增殖和PDGF-A的表达  

Interferon-γ inhibited the proliferation of smooth muscle cells and expression of PDGF-A induced by angioplasty in rabbit abdominal aorta

作　　者：陈晓春[1] 陈凤荣[1] 黎洋 韩启德[1] 

机构地区：[1]北京医科大学第三医院血管医学研究所

出　　处：《中国病理生理杂志》1998年第3期230-234,共5页Chinese Journal of Pathophysiology

基　　金：卫生部基金

摘　　要：目的和方法：采用体外细胞培养，进行氚标胸腺嘧啶核苷（３Ｈ－ＴｄＲ）掺入和原位杂交。结果：发现γ－干扰素（ＩＦＮ－γ）能抑制体外培养新西兰兔血管平滑肌细胞（ＶＳＭＣ）的ＤＮＡ合成和血小板衍化生长因子Ａ链（ＰＤＧＦ－Ａ）的基因表达；整体实验中，用４ＦＦｏｇａｒｔｙ导管气囊拉伤新西兰兔的腹主动脉内皮后，随即连续肌注ＩＦＮ－γ（１６５万Ｕ·ｋｇ１·ｄ１）３天，结果发现肌注ＩＦＮ－γ能明显抑制受损部位ＶＳＭＣ的３Ｈ－ＴｄＲ的标记率及ＰＤＧＦ－Ａ的表达。结论：ＩＦＮ－γ对血管成形术引起的血管挛缩有一定的舒张作用。AIM and METHODS: 3 H-thymidine incoporation and in situ hybridization with PDGF-A cDNA probe were performed in cultured smooth muscle cells and restenosis model after angioplasty.RESULTS:Interferon-γ inhibited vascular smooth muscle cell DNA synthesis and the expression of PDGF-A. In vitro experiment showed that intramuscular injection of interferon-γ for three days decreased the labeling rate of 3 H thymidine and the mRNA of PDGF-A.CONCLUSION:The interferon-γ diminished the vascular constriction induced by injury, and had some vascular relaxation effects.

关 键 词：血管成形术 血管平滑肌细胞 干扰素类Ⅱ型 血小板-衍化生长因子 PDGF-A IFN-Γ 

分 类 号：R654.3[医药卫生—外科学]