高效反相液相色谱测定致龋菌DNAG+Cmol%  

Determination of DNA G+C mol% Using Reversed-phase High-performance Liquid Chromatography for the Identification of Cariogenic Streptococci

作　　者：刘艳玲[1] 冯希平 潘瑛[1] 

机构地区：[1]上海第二医科大学附属第九人民医院口腔预防科,200011

出　　处：《口腔医学》2001年第1期3-5,共3页Stomatology

基　　金：上海市科学技术发展基金资助项目!编号 :97ZB14 0 2 1

摘　　要：目的 :鉴定变形链球菌和远缘链球菌临床分离株。方法 :采用苯酚氯仿混合法提取纯化 46株变链菌和远缘链球菌分离株的DNA ,采用高效反相液相色谱法测定细菌DNAG +Cmol%。结果 :30株变链菌和 16株远缘链球菌分离株的DNAG +Cmol%分别为 36 2 2～ 38 81和 44 5 7～ 45 77,两者差异大于 5 % ,并与国际参考菌株变链菌Ingbritt和远缘链球菌 6 715的G+Cmol%一致。结论 :DNAG +Cmol%测定可作为细菌分类学的重要指标。Objective:To identify the species of Streptococcus mutans and Streptococcus sobrinus clinical isolates.Methods:Purified bacterial DNA was hydrolysed by perchloric acid;and the DNA G+C mol% of clinical isolates was determined using reversed-phase high-performance liquid chromatography.Results:The G+C content of S.mutans isolates ranged from 36.22～38.81 moles%,and that of S.sobrinus isolates from 44.57～45.77.The results were comparable with those obtained with the thermal method.Conclusion:The G+C content of DNA is one of the important criteria in microbial taxonomy.

关 键 词：远缘链球菌 高效反相液相色谱 G+Cmol% 变形链球菌 分离 口腔细菌学 

分 类 号：R780.2[医药卫生—口腔医学]