低剂量^(147)Pm内照射诱发小鼠细胞遗传突变抗性的研究  

Effects of low doses ^(147) Pm internal irradiation against hereditary mutation in mouse cells induced by high dose of radiation\

作　　者：杨占山[1] 杨淑琴[1] 朱寿彭[1] 

机构地区：[1]苏州大学放射医学系,江苏215007

出　　处：《中华放射医学与防护杂志》2001年第2期114-116,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家核工业科学基金资助项目!(7196 6 2 0 1)

摘　　要：目的　研究低剂量1 4 7Pm内污染对红细胞和淋巴细胞遗传突变的影响。方法　应用骨髓和血液嗜多染与正常染红细胞微核检测以及骨髓淋巴细胞染色体畸变分析观察。结果　与正常对照组比较 ,18 5kBq g体重1 4 7Pm内污染可引起红细胞微核细胞率和淋巴细胞染色体畸变率明显增多 (P <0 0 5或 0 0 1)。然而 ,预先应用 0 37,3 7和 37Bq g体重的1 4 7Pm内污染动物 ,3d后注入18 5kBq g体重的1 4 7Pm ,红细胞微核细胞率以及淋巴细胞染色体畸变率则显著低于单纯高剂量1 4 7Pm组 (P <0 .0 5或 0 0 1) ;并且某些细胞突变与对照组比较已无显著性差异 (P >0 .0 5 )。结论　低剂量1 4 7Pm内污染预处理 ,可在较长时间内诱导红细胞和淋巴细胞的辐射适应性反应 :1 4 7Pm内污染诱导的细胞适应性反应具有较宽的剂量范围 ;与淋巴细胞染色体畸变比较 。Objective\ To investigate the effects of low doses 147 Pm internal irradiation against hereditary mutation in mouse cells induced by subsequent high dose of 147 Pm irradiation. Methods\ The frequencies of micronucleated polychromatic erythrocytes (mn PCE) micronucleated normochromatic erythrocytes (mn NCE) and chromosome aberration in bone marrow and peripheral blood cells were observed. Results\ The frequencies of nm PCE、mn NCE and chromosome aberration induced by high dose of 147 Pm irradiation showed a highly significant increase over the control at 24 h after irradiation ( P <0.05 or 0.01).However, the frequencies in cells pre exposed to 0.37 37 Bq/g of 147 Pm were decreased significantly ( P <0.05 or 0.01),as compared with those of the group without pre exposure. Conclusion\ Low doses of in vivo 147 Pm β irradiation can induce a radioadaptive response of the lymphocytes and erythroblasts.The induction of the adaptive response has a wide dose range.The mn PCE and mn NCE are more sensitive indexes for revealing biological effects of radiation as compared with the chromosome aberration.

关 键 词：^147PM 红细胞 淋巴细胞 微核 染色体畸变 细胞遗传学 适应性反应 

分 类 号：R817[医药卫生—影像医学与核医学]