从rds小鼠视网膜克隆视网膜色素变性相关差异片段  

Cloning the differentially expressed genes in the retina of rds mouse during the development of retinitis pigmentosa

作　　者：申煌煊[1] 张清炯[1] 邝志和[1] 肖学珊[1] 黎仁强[1] 

机构地区：[1]中山医科大学中山眼科中心眼遗传分子生物学实验室,广州510060

出　　处：《中华医学遗传学杂志》2001年第2期114-117,共4页Chinese Journal of Medical Genetics

基　　金：霍英东青年教师基金!(61 0 4 1 );广东省自然科学基金!(970 0 83);国家"863"计划!(Z1 9- 0 1 - 0 4 - 0 2 )&&

摘　　要：目的　从遗传性视网膜色素变性动物模型 rds小鼠中克隆视网膜色素变性发病过程中特异表达的基因。方法　应用差异显示技术 ,分析 rds小鼠发病过程中视网膜的 m RNA。对特异性表达的m RNA片段进行克隆测序。结果　在视网膜色素变性发病过程中 ,rds小鼠的视网膜存在着相当明显的基因表达差异。在所测序分析的 5个差异显示片段中 ,其中一个与 Gen Bank刚登录的功能未知的、从成年男性睾丸组织中克隆出来的 c DNA序列较高同源 (同一率为 86 % ) ,另外 4个为低同源序列。2 5天 rds小鼠高表达的一个差异片段 ,与 37天正常鼠表达而 rds小鼠不表达的另一个片段 ,长度相同 ,177个碱基只相差两个。结论　视网膜色素变性这类慢性病变 。Objective: To clone the differentially expressed genes in the retina of rds mouse (the animal model of congenital retinitis pigmentosa) during the disease development. Methods: The retinal mRNA of rds mouse during the development of retinitis pigmentosa was analyzed by the mRNA differential display. The differentilly expressed mRNA fragments were cloned and sequenced. Results: There was obvious difference of gene expression between rds mouse and the control during the development of retinitis pigmentosa. Five differentially expressed bands were cloned and sequenced. One of those had 86% identity (132/154) with the sequence of the human cDNA DKFZp434D1227 from adult testis in GenBank, which was submitted lately (15-Oct-1999) and without much information. The other had lower identity with the sequeces in GenBank. A highly expressed clone in the rds mouse on postnatal day 25 had the same length as another clone in the normal on postnatal day 37, which was not expressed in the rds mouse on day 37. The sequences of the two clones were identical in all but two base pairs. Conclusion: These results indicate that there are a lot of novel differentially expressed genes in the chronic processing diseases, such as retinitis pigmentosa.

关 键 词：MRNA 差异显示 视网膜色素变性 RDS小鼠 

分 类 号：R774.1[医药卫生—眼科]