酵母菌类金属硫蛋白的分离、纯化及性质鉴定  

ISOLATION, PURIFICATION AND CHARACTERIZATION OF METALLOTHIONEIN-LIKE PROTEIN FROM SACCHAROMYCES CEREVISIAE

作　　者：李明春[1] 李登文[1] 胡国武[1] 侯文强[1] 邢来君[1] 

机构地区：[1]南开大学微生物学系,天津300071

出　　处：《菌物系统》2001年第2期214-221,共8页Mycosystema

摘　　要：经过对酵母菌的金属抗性试验和培养条件试验，确定了高产类金属硫蛋白的酿酒酵母菌株Cu-21（Saccharomyces cerevisiae Cu-21）及其最佳培养条件。对该菌株的菌体蛋白进行SephadexG-100、DEAE-52结合的两次柱分离纯化，获得了类金属硫蛋白的三个亚型，蛋白性质鉴定结果表明含三个亚型、分子量小、富含半胱氨酸和金属元素，具有典型的巯基吸收特性。Saccharomyces cerevisiae Cu-21 was selected and its culture conditions were determinated. The proteins were isolated and purified by Sephadex G-100 and subsequent DEAE-52, and three types of Cu-MTs were found. The purified proteins were considered to be MTs-like protein by their characters of low-molecular weight, high cysteine and metal content and special absorbency spectrums.

关 键 词：类金属硫蛋白 分离纯化 酿酒酵母 

分 类 号：Q936[生物学—微生物学]