快速检测尖锐湿疣组织中人乳头瘤病毒6型和11型DNA的研究  

A Rapid Detection of Human Papillomavirus 6, 11 DNA in Condyloma Acuminatum

作　　者：李明发[1] 麻晓迁[1] 谢维[1] 单祥年[1] 蒋清[1] 茅一萍[1] 刘季和[2] 常宝珠[2] 孙建方[2] 杨志坚[3] 

机构地区：[1]南京铁道医学院生物学教研室,南京210009 [2]中国医学科学院皮肤病研究所 [3]江苏省肿瘤防治研究所病毒室

出　　处：《中国病毒学》1993年第1期71-75,共5页Virologica Sinica

基　　金：江苏省卫生厅资助课题

摘　　要：本文应用聚合酶链反应技术对30例人生殖器尖锐湿疣组织中HPV6,11 DNA的存在进行了检测研究。结果发现HPV6,HPV11 DNA的阳性率分别为60％,90％HPV6,HPV11 DNA双重检出率为53.3％,总阳性率达96.7％,本文结果证实HPV6,11的感染和尖锐湿疣的发生密切相关。本文检测方法具有快速,灵敏、特异性强的优点,为HPVDNA的检测及其分型研究提供了有效的手段。作者还对两例女阴假性湿疣进行PCR扩增,结果似乎不支持女阴假性湿疣的HPV感染的病因学。The polymerase chain reaction (PCR) was applied to identify the human papillomavirus (HPV) 6 and 11 DNA in the spcimens from 30 patients with condyloma acuminatum. The positive percentage of HPV6, HPV11 DNA was 60%, 90%, respectively. 53.3% samples showed the presence of both HPV6 and HPV11 DNA. The total positive percentage was 96.7%. Our result supported the data presented previously that the HPV6 and HPV11 are strongly associated with condyloma acuminatum. The PCR proved to be a rapid, sensitive, highly specific for detection and typing of HPV DNA. The negative amplification result of two cases of atypical condyloma could contribute to the explanation of its etiology.

关 键 词：生殖器尖锐湿疣 女阴假性湿疣 人乳头瘤病毒 聚合酶链反应 快速检测 

分 类 号：R752.53[医药卫生—皮肤病学与性病学] R446.5[医药卫生—临床医学]