子宫颈上皮内瘤变端粒酶活性的研究  

Telomerase activity in cervical intraepithelial neoplasia

作　　者：王淑珍[1] 孙建衡[1] 张伟[2] 金顺钱[2] 王洪平[2] 李茉[2] 金玉生[2] 曲萍[2] 刘毅[2] 

机构地区：[1]中国医学科学院中国协和医科大学肿瘤医院妇瘤科,北京100021 [2]中国医学科学院中国协和医科大学肿瘤医院肿瘤检测中心,北京100021

出　　处：《中华妇产科杂志》2001年第5期275-277,共3页Chinese Journal of Obstetrics and Gynecology

摘　　要：目的　探讨端粒酶激活在宫颈癌变过程中的意义及其作为病情监测方法和预测宫颈上皮内瘤变 (CIN)结局的可能性。方法　采用端粒重复序列扩增 聚合酶链反应 (PCR TRAP)方法检测了 6 4例CIN患者、2 1例宫颈癌患者、2 0例慢性宫颈炎患者及 15例正常宫颈妇女宫颈脱落细胞、宫颈活组织检查 (活检 )组织的端粒酶活性。结果　正常宫颈、慢性宫颈炎、CINⅠ级 (CINⅠ )、CINⅡ级(CINⅡ )、CINⅢ级 (CINⅢ )及宫颈癌患者的宫颈脱落细胞端粒酶的阳性表达率分别为 2 0 0 %、2 5 0 %、6 2 5 %、6 0 0 %、82 4%及 6 1 9% ;对应的活检组织端粒酶的阳性表达率分别为 2 6 7%、30 0 %、5 0 0 %、45 0 %、96 4%及 95 2 % ;随着宫颈病变的进展 ,端粒酶阳性表达率呈逐渐增高趋势(χ2 细胞 =16 2 8、χ2 组织 =36 98,P均 <0 0 5 )。CINⅠ、CINⅡ端粒酶阳性表达率比较 ,差异无显著性 (P细胞=0 2 4、P组织 =0 2 5 ) ;CINⅢ端粒酶阳性表达率高于CINⅠ、CINⅡ (P细胞 =0 0 3、P组织 =0 0 0 0 0 12 ) ;CINⅢ与宫颈癌活检组织端粒酶阳性表达率比较 ,差异无显著性 (P =0 0 5 ) ;宫颈脱落细胞与宫颈活检组织的端粒酶检测结果的对应性良好 (χ2 =46 4,P <0 0 5 )。结论　端粒酶激活与宫颈癌变的进程有关 。Objective To investigate the relationship between telomerase expression and the carcinogenesis of cervix, and the possibility of using telomerase as a marker to evaluate the natural history of cervical intraepithelial neoplasia (CIN) Methods Use the telomere repeat amplification protocol (TRAP) assay to detect cervical lesion in cervical scraping samples and cervical biopsy tissues Results Sixty two point five percent, 60 0 percent, 82 4 percent and 61 9 percent cervical scraping samples exhibited telomerase activity in CINⅠ , CINⅡ , CINⅢ and cervical cancer, respectively, compared 50 0 percent, 45 0 percent, 96 4 percent and 95 2 percent in cervical biopsy tissue In contrast, 20 0 percent and 26 7 percent in cervical scraping samples and cervical biopsy tissues of normal cervix With the development of cervical lesion, the expression of tolemerase activation was increasing ( P <0 05) There was no significant difference between CINⅠ and CINⅡ ( P >0 05) There were significant differences between CINⅢ and CINⅠ , CINⅡ ( P <0 05) Telomerase activity was coincidence between the cervical scraping samples and cervical biopsy tissues Conclusions These results seem to suggest that the telomerase expression may associate with the carcingenesis of cervix The telomerase activity assay of cervical scraping sample could be used as a marker to monitor and predicate the transit of CIN in clinical practice

关 键 词：子宫颈上皮内瘤样病变 端粒 末端转移酶 宫颈肿瘤 聚合酶链反应 

分 类 号：R737.33[医药卫生—肿瘤]