视网膜母细胞瘤中P^(16)基因蛋白表达的免疫组化研究  

Immunohistochemical studies on P^(16) and P^(53)genes protein expressions in retinoblastoma

作　　者：孙红[1] 于秀敏[1] 王利华[1] 马鲁新[1] 杜德利[1] 梁克勤[1] 王延军[1] 

机构地区：[1]山东省立医院,济南250021

出　　处：《山东医大基础医学院学报》2001年第2期65-68,共4页

摘　　要：目的 :研究视网膜母细胞瘤肿瘤组织中P16 蛋白的表达状况 ,并探讨它与RB病理分化程度、视神经浸润能力和预后之间的关系。方法 :采用免疫组化SABC法。结果 :17例正常视网膜组织中P16 蛋白表达阳性率明显高于 4 9例RB肿瘤组 (P <0 0 5) ;13例分化型RBP16 蛋白阳性表达率明显高于 36例未分化型RB(P <0 0 5) ;31例视神经未浸润组RBP16 阳性表达率明显高于 18例视神经浸润组 (P <0 0 5) ;RB存活组P16蛋白阳性表达率与死亡组差异无显著性 (P >0 0 5)。结论 :RB中可能存在P16 基因异常 ,所致的P16 蛋白失表达与RB的组织学分型和视神经浸润能力的获得有关 ,但尚不能认为与RB的预后有关。Objective:To detect the expression of P 16 gene protein in RB tumor and investigate the relationship between P 16 protein expression and the pathological cell type, invasion capacity of optic nerve and the prognosis of RB.Method:49 cases of primary RB tumors were examined by streptavidin-biotin-enzyme complex (SABC) immunohistochemical technique.Results:The positive expression rate of P 16 in normal retina of 17 cases was markedly higher than that in RB of 49 cases (P<0.05).The expression rate of P 16 in differentiated RB tissues of 13 cases was significantly higher than that in non-differentiated group of 36 cases (P<0.05);and it was markedly higher in the optic nerve non-infiltrated group of RB(31 cases) than in that of the optic nerve infiltrated group(18 cases)(P<0.05).But between the alive and dead group,the positive expression rate of P 16 had no significant difference statistically (P<0.05).Conclusion:Loss of P 16 protein expression is correlated with the poor pathological differenciated type and the acquisition of optic nerve infiltrating capacity of RB tumour cells.But it could't be considered to have relationship with the prognosis of RB.

关 键 词：视网膜母细胞瘤 P16基因 P53基因 免疫组织化学 

分 类 号：R739.71[医药卫生—肿瘤] R774.1[医药卫生—临床医学]