茵陈素对肺癌细胞增殖和细胞周期的影响  

Effects of 6,7-dimethoxycoumarins on Cell Proliferation and Cell Generation Cycle of Human Lung Carcinoma Cell in Vitro

作　　者：谭永忠[1] 戴国华[1] 李军[1] 

机构地区：[1]重庆市第三人民医院,重庆市400014

出　　处：《中国药房》2001年第5期267-268,共2页China Pharmacy

摘　　要：目的 :探讨茵陈素对肺癌细胞增殖和细胞周期的影响。方法 :应用光镜、四氮甲唑蓝 (MTT)方法和流式细胞术分析茵陈素对肺癌细胞形态学、生长和细胞周期的变化。结果 :茵陈素能抑制肺癌细胞的增殖 ,呈剂量依赖性 ,以160μg/ml抑制作用最明显 ,抑制率达52 4 %。80μg/ml时 ,S期和G2/M期细胞比例开始下降 ,细胞被阻滞于G0/G1 期 ,不能进入S期及G2/M期 ,增殖指数明显下降。结论 :茵陈素在体外对肺癌细胞具有抑制作用 ,通过抑制DNA合成 ,将细胞阻滞于G0/G1 期来抑制细胞增殖。AIM:To investigate the effects of 6,7-dimethoxycoumarins on the proliferation and the cell cycle of human lung carcinoma cell(PA)in vitro METHODS:The effects of 6,7-dimethoxycoumarins on the cytomorphology,growth and cell cycle of PA cells were evaluated by MTT assay and flow cytometry in vitro RESULTS:6,7-dimethoxycoumarins could inhibit the proliferation of PA cells in a dose-dependent manner The inhibition action of 6,7-dimethoxycoumarins plateaued at 160μg/ml(52 4% inhibition) The agent in dose of 80μg/ml led to a decline in the proportion of cells to those in G2/M phase,made the cells remain in G0/G1 phase and reduced the proliferation index(PI) of PA cells CONCLUSION:In vitro,6,7-dimethoxycoumarins can inhibit the proliferation of PA cells,which leads to arrest of the cells in G0/G1 phase by way of inhibiting DNA synthesis

关 键 词：6 7-二甲氧基香豆素 肺癌 抑制作用 细胞周期 药理 

分 类 号：R734.205[医药卫生—肿瘤] R979.1[医药卫生—临床医学]