导入大豆的外源DNA同工酶鉴定  

Identification of Exogenous DNA Introduced into Soybean

作　　者：刘昭军 雷勃钧 卢翠华 韩玉琴 李希臣 周思君 钱华 

机构地区：[1]黑龙江省农科院生物技术研究中心

出　　处：《黑龙江农业科学》1999年第5期19-21,共3页Heilongjiang Agricultural Sciences

摘　　要：本文采用双垂直板聚丙烯酰胺凝胶电泳的方法对海豆、虎林绿草豆的外源总ＤＮＡ导入后代用过氧化物同工酶酶谱的差异分析，结果表明：酶谱清晰，供体受体及后代在谱带上存在着差异及相关特性。从生化角度揭示了其遗传背景相关性，证明外源ＤＮＡ已经导入受体，并得到整合、表达。With the method of Double Vertical Boards Polyacrylamide Gel Electrophoresis, peroxidase isozyme patterns was analysed in variant progenies of soybean received exogenous DNA of Coastal Bean and Hulinlucaodou. The results showed that patterns of POD enzyme were clear, and there were differences and correlation between patterns of donor, receptor and progenies, and also showed their genetic resemblance in biochemistry, and suggested exogenous DNA had been introduced into receptor, and inserted into the genome of the receptor and expressed.

关 键 词：外源总DNA 过氧化物同工酶 酶谱分析 大豆 花粉管通道技术 

分 类 号：S565.1[农业科学—作物学] S336