体外rhIL-1β诱导髁状突软骨细胞HSP70的表达及其意义  

Expression and role of heat shock protein 70 on condylar chondrocytes after treated with rhIL-1β in vitro

作　　者：李松[1] 赵书芳[2] 金岩[2] 

机构地区：[1]昆明医学院口腔系,650031 [2]第四军医大学口腔医学院

出　　处：《现代口腔医学杂志》2001年第3期161-163,I001,共4页Journal of Modern Stomatology

基　　金：云南省应用基础研究基金项目!( 99C0 0 0 8R)

摘　　要：目的　明确热休克蛋白 70 (HSP70 )在人重组白细胞介素 - 1(rhIL - 1β)刺激下对下颌骨髁状突软骨细胞的保护作用。方法　通过原代细胞培养获得兔髁状突软骨细胞 ,在生长良好的第三代软骨细胞中分别加入不同浓度的rhIL - 1β ,并利用原位杂交及免疫组织化学技术检测rhIL - 1β处理软骨细胞后HSP70的表达变化。 结果正常髁状突软骨细胞胞浆内有HSP70蛋白弱表达 ,rhIL - 1β(10ng/ml)作用 12h后HSP70出现表达升高 ,部分细胞核内也可见表达 ,48h后信号开始减弱。HSP70mRNA的表达与蛋白表达基本一致。结论　一定浓度的rhIL -1β刺激髁状突软骨细胞可诱导HSP70的转录及蛋白合成 ,提示HSP70可能在骨关节炎症损伤中对软骨细胞起到保护作用。Objective Heat shock proteins are extremly conservative proteins that are expressed in prokaryotes and eukuayotes.HSP70 protein is an important one of HSPs,which play a protective role for cells in stress.In this study,the protective role of HSP70 was discussed on condylar chondrocytes.Methods The expressions of HSP70 protein and mRNA on chondrocytes were detected by immunohistochemical staining and in situ hybridization before and after treated with recombinant human interleukin-1β in vitro.Results The results showed that the over expression of HSP70 protein and mRNA were localized in the cytoplasm and some nuclei.The highest level of expression was found in the group treated after 24 hours,and it began to decline 48 hours later.Conclusion These results suggest that rhIL-1β can directly stimulate HSP70 expression,which may protect the condylar chondrocytes from injury.

关 键 词：颞下颌关节 软骨细胞 骨关节炎 热休克蛋白70 RHIL-1Β 

分 类 号：R782.6[医药卫生—口腔医学]