血脑屏障可逆性开放增强^(99m)Tc-TRODAT-1脑摄取的初步研究  

Reversible opening of the blood-brain barrier by mannitol increases brain uptake of ^(99m)Tc-TRODAT-1

作　　者：孙文善[1] 朱承谟[1] 吕中伟[1] 吴锦昌[2] 

机构地区：[1]上海第二医科大学附属瑞金医院,上海200025 [2]苏州大学附属第二医院,苏州215004

出　　处：《核技术》2001年第5期337-340,共4页Nuclear Techniques

摘　　要：为探索应用血脑屏障可逆性开放是否可应用于增强99mTc -TRODAT - 1脑摄取 ,把 36只大鼠分为正常组和开放组 ,分别给予左侧股静脉注射 0 .9%生理盐水和 2 0 %甘露醇 ;再经尾静脉注射 0 .2mL99mTc -TRODAT - 1( 7.4MBq) ,比较两组不同时间99mTc -TRODAT - 1的脑摄取。将SD大鼠分为甘露醇组和甘露醇 +GBR12 90 9组 ,观察甘露醇开放血脑屏障是否影响99mTc -TRODAT - 1的特异性结合。结果表明 ,开放组大鼠经注射甘露醇后 ,全脑和纹状体摄取在 2、30、60min时均比对照组增高 ,纹状体 小脑在 2min时也明显增高 ;甘露醇 +GBR12 90 9组纹状体 小脑值显著低于甘露醇组 ,表明甘露醇注射后并不影响99mTc -TRODAT - 1与DAT的结合特异性。说明血脑屏障的可逆性开放可应用于增加99mTc标转运蛋白显像剂进脑量的研究 ,以改善图象质量。To determine whether the reversible opening of blood-brain barrier is able to increase the brain uptake of 99m Tc-TRODAT-1; Adult Sprague-Dawley rats were infused with mannitol via femoral vein at rates 0.25mL/kg·s for opening blood-brain barrier,GBR12909 was used to characterize the pharmacological specificity of 99m Tc-TRODAT-1 for dopamine transporters after infusion of mannitol. After infusion of mannitol,the brain uptake of 99m Tc-TRODAT-1 showed a dramatic increase,but no effect on striatum/cerebellum and pharmacological specificity of 99m Tc-TRODAT-1. This study shows that the reversible opening of blood-brain barrier with mannitol may be a useful approach to improve brain uptake of 99m Tc-TRODAT-1 and other 99m Tc-labeled specific receptor imaging agents.

关 键 词：血脑屏障可逆性开放 多巴胺转运蛋白 TRODAT-1 锝99M标记 脑摄取 放射治疗 脑肿瘤 药物治疗 脑显像剂 

分 类 号：R739.91[医药卫生—肿瘤] R730.55[医药卫生—临床医学]