南荻遗传转化系统的建立及转基因植株的获得  

Establishment of Genetic Transformation System for Miscanthus Sacchariflorus and Obtained of Its Transgenic Plant

作　　者：易自力[1] 周朴华[1] 储成才 李祥[1] 田文忠[2] 王力[2] 曹守云[2] 唐柞舜 

机构地区：[1]湖南农业大学生物技术系,长沙410128 [2]中国科学院遗传研究所,北京100101

出　　处：《高技术通讯》2001年第4期20-24,共5页Chinese High Technology Letters

摘　　要：建立了南荻愈伤组织高频诱导与再生系统及其转化愈伤组织的筛选系统 ,并通过本研究所建立的基因枪转化系统 。The initiation and regeneration system for embryogenic callus of Miscanthus sacchariflorus were established at very high frequency. Potato proteinase inhibitor II ( pin II) gene were introduced into the callus of Miscanthus sacchariflorus and some transgenic plants were obtained by the particle bombardment transformation system. PCR and Southern analysis showed that target genes were integrated into the genome of these transgenic plants.

关 键 词：南荻 基因枪轰击 马铃薯 蛋白酶抑制基因 愈伤组织 转基因植株 

分 类 号：Q943[生物学—植物学]