筑巢式RT-PCR检测慢性粒细胞白血病残留细胞  

RESIDUAL CELLS DETECTION IN CHRONIC MYELOCYTIC LEUKEMIA WITH RT-PCR

作　　者：邱运树[1] 焦颖军 

机构地区：[1]山西省儿童医院,山西太原030013 [2]山西省太原市杏花岭区计划生育服务站,山西太原030013

出　　处：《白血病．淋巴瘤》2001年第2期98-99,共2页Journal of Leukemia & Lymphoma

摘　　要：目的 :检测慢性粒细胞白血病残留细胞。方法 :应用 RT- PCR方法对 2 0例 (其中 6例为异基因骨髓移植术后 )慢性粒细胞白血病患者进行血或骨髓的 bcr/ abl m RNA检测。结果 :2 0例初发时 Ph染色体阴性 1例 ,阳性 19例 ,2 0例均检出 bcr/ abl基因扩增产物。全部病例进行连续性监测发现 ,6例异基因骨髓移植患者 5例最终 Ph染色体及 bcr/ abl基因转阴 ,1例持续阳性并检出两类 (16 8bp或 93bp) bcr/ abl基因。结论 :RT- PCR是当前检测慢性粒细胞白血病残留细胞比较可靠的方法。Objective: To detect the residual cells in chronic myelocytic leukmia. Methods:RT-PCR method was used to study the bcr/abl mRNA in 20 cases chronic leukemia (including 6 cases allogeneic bone marrow transplantation).Results:At the beginning, chromosome Ph was negative in 1,and positive in19 of 20 cases. All cases expressed bcr/abl gene. Continue monitoring found that chromosome Ph had become into negtive in 5,and still positive in 1 of 6 allogeneic bone marrow transplantation patients. Conclution: RT-PCR is a useful method to study the residual cells in choronic myelocytic leukemia.

关 键 词：慢性粒细胞白血病 RT-PCR 残留细胞 

分 类 号：R733.72[医药卫生—肿瘤]