以降钙素基因高度甲基化为分子基因标志检测白血病微量残留病  

Detecting minimal residual disease of leukemia with hypermethylation of the calcitonin gene as molecular genetic marker

作　　者：唐义平[1] 邓承祺[2] 杜琼[3] 李戈[4] 张晋琳[1] 缪世锟[1] 

机构地区：[1]四川省人民医院血液科,成都610071 [2]华西医科大学附属第一医院血液科,成都610041 [3]四川省人民医院检验科,成都610071 [4]四川省人民医院儿科,成都610071

出　　处：《医学科技》2001年第1期6-9,共4页

摘　　要：目的:探讨以降钙素基因高度甲基化为白血病克隆的分子基因标志,检测白血病微量残留病,并预测预后的可能性。方法:用限制性内切酶消化 DNA,应用多聚酶链反应(PCR)技术检测29例急性白血病和8例慢性粒细胞白血病急变患者的降钙素基因的甲基化程度。对降钙素基因高度甲基化阳性者,以降钙素基因高度甲基化为白血病克隆的分子基因标志,应用PCR技术定期追踪检测骨髓微量残留病。结果:20例白血病完全缓解后均存在微量残留病。完全缓解后微量残留病持续阳性或转阴后再次出现阳性,提示将发生骨髓复发,能提早2～11个月预示疾病的复发。微量残留病较早转阴并持续长期阴性者可能获得长期生存。结论:以降钙素基因高度甲基化为白血病克隆的分子基因标志,应用PCR 技术能起到监测白血病骨髓微量残留病的作用,为预测白血病的预后开辟了一条新途径。Objective:To explore whether detecting minimal residual disease of leukemia with hyper-methylation of the calcitonin gene as molecular genetic marker of leukemic clone to predict the prognosis.Methods: Polymerase chain reaction in combination with digestion of DNA with Hpa Ⅱ was used to ex-amine the methylation patterns of the calcitonin gene in 29 cases with acute leukemia and 8 cases withtransformation of chronic myeloid leukemia. By using polymerase chain reaction, minimal residual diseasewas longitudinally detected in patients which were positive for hypermethylation of the calcitonin gene withhypermethylation of the calcitonin gene as molecular genetic marker. Results: All of patients with leuke-mia had minimal residual disease after complete remission. Bone marrow relapse was occurred soon whenminimal residual disease persisted or reappeared. It may predict bone marrow relapse ahead of two toeleven months. The patients which got early negative for minimal residual disease and were persistent neg-ative may acquire prolonging survival. Conclusion: Minimal residual disease of leukemia may be moni-tored by using polymerase chain reaction with hypermethlation of the calcitonin gene as molecular geneticmarker for leukemic clone. It may prove useful in predicting prognosis of leukemia.

关 键 词：微量残留病 降钙素基因 高度甲基化 多聚酶链反应 白血病 

分 类 号：R733.7[医药卫生—肿瘤]