NF-κB活化在IL-1β诱导的小鼠肾小球系膜细胞表达IL-6中的作用  

Role of nuclear factor κB on the expression of interleukin-6 in mouse mesangial cells induced by interleukin-1β

作　　者：许韩师[1] 叶任高[1] 杨琼琼[1] 孙林[1] 杨念生[1] 李幼姬[1] 曾丽霞[1] 

机构地区：[1]中山医科大学附属第一医院肾内科卫生部肾脏病临床研究重点实验室,广东广州510080

出　　处：《中国病理生理杂志》2001年第5期428-430,共3页Chinese Journal of Pathophysiology

基　　金：中山医科大学 2 11工程项目基金!资助 (No .9815 0 )

摘　　要：目的 :研究NF -κB在rhIL - 1β刺激引起的体外培养的鼠肾小球系膜细胞表达IL - 6中的作用。 方法 :NF -κΒ活性检测采用电泳迁移率改变法 (EMSA) ,IL - 6mRNA表达采用逆转录 /聚合酶链反应 (RT/PR)检测 ,培养上清IL - 6蛋白含量采用ELISA检测。结果 :rhIL - 1β刺激肾小球系膜细胞时 ,在上调IL - 6蛋白和基因表达的同时亦激活NF -κB ,而且这种上调作用可被NF -κB特异性抑制剂PDTC所阻抑。结论 :IL - 1β诱导鼠肾小球系膜细胞表达IL - 6是通过NF -κB调控 ,NFAIM: To investigate the regulatory role of nuclear factor κB (NF-κB) in the expression of interleukin-6 in mesangial cells (MC) induced by interleukin-1β.METHODS: Activation of NF-κB was measured by electrophoresis mobility shift assay (EMSA). RT/PCR and ELISA were used to detect IL-6 mRNA expression and IL-6 production, respectively.RESULTS: rhIL-1β could rapidly stimulate the activation of NF-κB in MC, and increase the expression of IL-6 mRNA and protein. PDTC, one of the inhibitor of NF-κB, could inhibit the expression of IL-6 in mRNA and protein in MC stimulated by rhIL-1β.CONCLUSION: IL-6 expression induced by IL-1β may be regulated by NF-κB in MC, NF-κB may modulate the immune-inflammatory reaction in glomerular disease.

关 键 词：白细胞介素1 白细胞介素6 NF-KB 肾小球肾炎 小鼠 肾小球系膜细胞 

分 类 号：R692.31[医药卫生—泌尿科学] R392.32[医药卫生—外科学]