CD40在人腹膜间皮细胞的表达  

Expression and regulation of CD40 on human peritoneal mesothelial cells

作　　者：阳晓[1] 叶任高[1] 杨琼琼[1] 孔庆瑜[1] 汪涛[1] 余学清[1] 

机构地区：[1]中山医科大学第一附属医院卫生部肾脏病临床研究重点实验室,广东广州510080

出　　处：《中国病理生理杂志》2001年第5期448-450,共3页Chinese Journal of Pathophysiology

基　　金：卫生部重点科研基金!资助 (No .970 40 2 2 8) ;中山医科大学 2 11工程重点科研基金!资助 (No .9815 1)

摘　　要：目的 :对人腹膜间皮细胞CD40的表达及其调节因素进行初步探讨。方法 :从CAPD患者的透出液中分离、培养腹膜间皮细胞 ,用IFN -γ、TNF -α、IL - 1、LPS刺激 2 4h ,通过流式细胞仪 (FACS)检测分析腹膜间皮细胞CD40、CD40L及ICAM - 1的表达。结果 :腹膜间皮细胞结构性表达少量的CD40 ;IFN -γ可显著增加腹膜间皮细胞表面CD40蛋白的表达 ,而TNF -α、IL - 1、LPS对腹膜间皮细胞表面CD40蛋白的表达无显著影响。未见间皮细胞表达CD40L。IFN -γ、TNF -α、IL - 1、LPS对间皮细胞ICAM - 1表达均有显著增强作用。IFN -γ增强ICAM - 1表达作用显著高于TNF -α、IL - 1、LPS ,间皮细胞CD40表达强度与ICAM - 1呈显著正相关。结论 :人腹膜间皮细胞可功能性表达CD40。AIM: To determine whether human peritoneal mesothelial cells express CD40. METHODS: Human peritoneal mesothelial cells (HPMC) were harvested from spent peritoneal dialysis effluent and maintained under defined in vitro conditions. Expression of CD40, CD40L and intercellular adhesion molecule-1 (ICAM-1) on HPMC under normal culture or stimulation with interferon-γ (IFN-γ), tumor necrosis factor-α(TNF-α), interleukin(IL)-1 and LPS were detected by FACS analysis. The relationship between CD40 expression and ICAM-1 expression on HPMC was analyzed.RESULTS: HPMC cultured in vitro expressed CD40 constitutively. The surface expression of CD40 was markedly up-regulated following stimulation with IFN-γ, but not with TNF-α, IL-1 and LPS. CD40L expression on HPMC was not detected. The expression of ICAM-1 on HPMC was significantly increased by stimulation with IFN-γ, TNF-α, IL-1, LPS, respectively; the most effective inducer on ICAM-1 expression was IFN-γ. The CD40 expression co-localizes with the expression of ICAM-1 and there was positive correlation between CD40 and ICAM-1 expression on HPMC. CONCLUSION: HPMC functionally expressed CD40.

关 键 词：腹膜间皮细胞 CD40抗原 细胞粘着分子 流式细胞术 

分 类 号：R572.2[医药卫生—消化系统]